RCIPL
Repositório Institucional do Politécnico de Lisboa
Entradas recentes
Avaliação da eficácia do smartwatch na análise eletrocardiográfica: derivação DI vs derivação compatível obtida no smartwatch
Publication . Neto, Sara Samji; Coelho, André; Fonseca, Virgínia
Introdução: A crescente utilização de Smartwatches (SW) com capacidade de registar sinais elétricos cardíacos tem aumentado. São escassos os dados quanto à eficácia de modelos Smartwatch Samsung. Este estudo centra-se no Samsung Galaxy Watch 4, avaliando se o registo obtido é semelhante à derivação DI obtida através do Eletrocardiograma (ECG) quando comparados os parâmetros eletrocardiográficos. Objetivos: Avaliar a eficácia do registo do sinal elétrico cardíaco realizado no Samsung Galaxy Watch 4 por comparação com a derivação eletrocardiográfica – DI – realizada num eletrocardiógrafo.
Metodologia: Estudo transversal, descritivo-correlacional, com uma amostra de 40 utentes. Realizaram-se simultaneamente os dois registos. Foram obtidas as variáveis quantitativas (Frequência Cardíaca (FC), Intervalo PQ e Duração do Complexo QRS) e qualitativas (Ritmo Cardíaco, Morfologia do Complexo QRS, Polarização da Onda P e Onda T, presença de sístoles prematuras supraventriculares e ventriculares), em ambos os registos. A análise estatística incluiu correlações de Bland-Altman e o teste de McNemar. Resultados: As médias da FC: 73,0 ± 10,8 bpm (ECG) e 75,8 ± 11,1 bpm (SW); do intervalo PQ: 161,5 ± 26,5 ms (ECG) e 162,7 ± 27,5 ms (SW); e da duração do Complexo QRS: 87,2 ± 13,3 ms (ECG) e 88,2 ± 15,1 ms (SW). Concordâncias foram r = 0,997 para FC, r = 0,993 para o intervalo PQ e r = 0,985 para a duração do complexo QRS, com viés médio reduzido. Não foram evidenciadas diferenças significativas nas variáveis qualitativas, com apenas um falso positivo de Fibrilhação Auricular no SW. Conclusão: O SW Samsung Galaxy Watch 4 apresenta elevada sensibilidade quando comparado com o ECG. Embora o uso deste SW não substitua o exame gold standard, apresenta potencial para ser integrado como ferramenta de rastreio, reforçando a necessidade de ocorrer uma validação clínica posterior dos algoritmos do SW.
A call for preregistration of in vitro research: pregistration as a tool to improve reproducibility and transparency
Publication . Heinl, Céline; Franco, Nuno H.; Bert, Bettina; Siewert, Katherina; Movia, Dania; Aruçi, Edlira; Ladeira, Carina; Sanin, Ahmed Y.; Neuhaus, Winfried; Kahlert, Ulf D.; Ghosh, Manosij; Thomas, Aurélie; Sotiropoulos, Athanassia
Biomedical research, like any other research field, is vulnerable to problems in its planning, execution, documentation, analysis, interpretation, and reporting. These issues can impede the reproducibility of research findings and hinder translation into clinical practice. In addition, there is growing societal pressure to hasten the shift to animal-free research methods. To alleviate the ethical dilemma of using animals in research, the 3Rs principle (replace, reduce, refine) calls for reducing or replacing such experiments wherever possible. Nevertheless, scientific and biomedical research, as well as safeguarding human and environmental health, continue to depend on animal experiments. To apply the 3Rs principles without compromising scientific progress and safety standards, it is imperative to ensure that in vitro methods to replace animal experiments are robust and reliable.
Microwave heating as an alternative to conventional drying ovens for histological section adhesion: evaluation across different tissue types
Publication . Anselmo, Beatriz; Laranjeiro, Marisa; Novo, Maria Manuela; Ferro, Amadeu José Borges
Adequate adhesion of histological sections to glass slides is essential for reliable microscopic evaluation and accurate clinical diagnosis. Conventionally, section adhesion is achieved using drying ovens, a method known for its effectiveness but requiring high energy consumption and substantial laboratory space. Microwave oven heating has emerged as a potential alternative, offering faster processing and lower operational costs; however, its effectiveness for slide adhesion has not been systematically validated across different tissue types. This study aimed to evaluate the feasibility of microwave heating at 8000W as an alternative to conventional oven drying for histological section adhesion.
Contraste em microscopia eletrónica de transmissão: as alternativas emergentes ao acetato de uranilo (revisão sistemática)
Publication . Correia, Sara; Pipio, Eva; Santos, Raquel; Pinto, Andreia; Ferro, Amadeu
Transmission electron microscopy (TEM) is a key technique for nanometric ultrastructural analysis in biomedical research. Uranyl acetate (UA) has long been the contrast agent of choice due to its high electron density and affinity for cellular components. However, UA is a radioactive, nephrotoxic, potentially carcinogenic, and subject to strict regulation by the European Chemicals Agency, prompting the need for safer alternatives. This systematic review aimed to evaluate whether non-radioactive substitutes for UA preserve ultrastructural image quality while improving laboratory safety.
Carcinogenicity of tris(chloropropyl) phosphate, butyraldehyde, and cumyl hydroperoxide
Publication . Lachenmeier, Dirk W.; Arrandale, Victoria H.; DeMarini, David M.; Ruksha, Tatiana; Abdallah, Mohamed A-E; Bettini, Giuliano; Ishii, Yuji; Ladeira, Carina; Pi, Jingbo; Rossner, Pavel; Ryan, Kristen R.; Stefanska, Barbara; Van Gerwen, Maaike; Venier, Marta; Conti, Aline de; Facchin, Caterina; Kunzmann, Andrew T.; Madia, Federica; Pasqual, Elisa; Wedekind, Roland; Al Nahas, Aline; Coutaz-Repland, Seyederoya; Ohene-Agyei, Phyllis; Suonio, Eero; Mattock, Heidi; Benbrahim-Tallaa, Lamia; Schubauer-Berigan, Mary K.
In March 2026, a Working Group of 12 scientists from nine countries met at the International Agency for Research on Cancer (IARC) in Lyon, France, to finalise their evaluation of the carcinogenicity of tris(chloropropyl) phosphate (TCPP), butyraldehyde, and cumyl hydroperoxide. TCPP was classified as “probably carcinogenic to humans” (Group 2A) based on the combination of “sufficient” evidence for cancer in experimental animals and “strong” mechanistic evidence in human primary cells. Butyraldehyde was classified as “possibly carcinogenic to humans” (Group 2B) based on “sufficient” evidence for cancer in experimental animals and on “strong” mechanistic evidence in experimental systems. Cumyl hydroperoxide was classified as “possibly carcinogenic to humans” (Group 2B) based on “strong” mechanistic evidence in human primary cells and experimental systems. These assessments will be published in Volume 141 of the IARC Monographs.