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Advisor(s)
Abstract(s)
Genotoxicity is an important information that should be included in human biomonitoring programs design. Cytogenetic methods are usually laborious and time-consuming; therefore, the development of new molecular methods is an added value. This study aimed to evaluate if the molecular profile of previously frozen whole blood acquired by Fourier Transform Infrared (FTIR) spectroscopy allows for to assessment of genotoxicity in occupational exposure to antineoplastic drugs in hospital professionals, as obtained by the lymphocyte cytokinesis block micronucleus (CBMN) assay.
Description
This work was supported by Instituto Politécnico de Lisboa under grant IDI&CA/IPL/ 2017 GenTox ESTeSL and by Fundação para a Ciência e a Tecnologia, Portugal, under grant DSAIPA/DS/ 0117 2020. The human biomonitoring had financial support given by the Portuguese Authority of Working Conditions (Project reference 036 APJ/09).
Keywords
Molecular profile FTIR-spectroscopy Genotoxicity Cytokinesis-blocked micronucleus assay Frozen blood Antineoplastics IDI&CA/IPL/2017/GenTox/ESTeSL FCT_DSAIPA/DS/0117/2020 ACT_036APJ/09
Pedagogical Context
Citation
Ladeira C, Araújo R, Ramalhete L, Teixeira H, Calado CR. Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs: a comparison with cytokinesis-block micronucleus assay results. In: 51st European Environmental Mutagenesis and Genomics Society (EEMGS) & 27th Spanish Environmental Mutagenesis and Genomics Society (SEMA), Málaga (Spain), May 15-18, 2023.