RCIPL
Repositório Institucional do Politécnico de Lisboa
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Factors associated with the recurrence of intermittent exotropia and reoperations in the long term
Publication . Lino, Pedro; Aguiar, Pedro; Cunha, João Paulo
Purpose: To evaluate long-term reoperation risk after bilateral lateral rectus (BLR) recession for intermittent exotropia (IXT) in a paediatric cohort, and to identify the perioperative factors associated with recurrence and reoperation. Methods: A retrospective observational cohort of 258 children with basic or divergence-excess IXT who underwent BLR recession at CUF Cascais Hospital between 2010 and 2020 was analysed. Clinical variables included age, age at surgery, pre- and immediate postoperative deviation angles, preoperative occlusion therapy, orthoptic treatment, binocular function, and initial surgical success (residual deviation <10 prism dioptres with fusion). Bivariate analyses were performed using t-tests or χ2 tests, and variables with p < 0.10 were entered into multivariable binary logistic regression to identify the independent predictors of reoperation. Model diagnostics included variance inflation factors (VIF), Hosmer-Lemeshow test, and area under the receiver operating characteristic curve (AUC). Significance was set at p < 0.05. Results: Reoperation was required in 11% of cases. Failed initial surgery (adjusted OR = 34.7; p < 0.001), larger preoperative deviation (adjusted OR per PD = 1.143; 95% CI 1.062-1.231; p < 0.001), larger immediate postoperative deviation (adjusted OR per PD = 0.822; 95% CI 0.766-0.881; p < 0.001), and older patient age (adjusted OR per year = 1.261; 95% CI 1.032-1.542; p = 0.024) were independently associated with reoperation. Orthoptic and occlusion therapies showed non-significant trends. Conclusion: In children undergoing BLR for IXT, initial surgical failure and deviation magnitude (pre- and immediate postoperative) are the strongest predictors of reoperation, with age having a modest additional effect. These findings emphasize precise surgical planning and the need for long-term follow-up.
Old molecules, new hope: a scoping review and bibliometric analysis of drug repurposing for lung cancer
Publication . Ragassi, Wellington Martins; Alves, Fernando Miguel; Lazo, Raul Edison; Tonin, Fernanda; Pontarolo, Roberto; Sari, Marcel Henrique; Ferreira, Luana Mota
Drug repurposing has gained prominence in oncology by enabling the investigation of approved drugs for new therapeutic purposes. In lung cancer, this strategy may reduce the time and costs associated with drug development. This study aimed to map the landscape of in silico, in vitro, in vivo, and clinical research on drug repurposing for lung cancer, while identifying key molecular targets and research gaps. A systematic search was conducted in PubMed, Embase, and Web of Science, following Joanna Briggs Institute and PRISMA-ScR guidelines. Two reviewers independently selected and extracted the data. A total of 58 studies, published between 2010 and 2024, mainly from the United Kingdom (19%) and the United States (17%), were included. Most studies used in vitro models (53%), followed by in vivo (31%) and in silico (16%), with frequent combinations of methods. The most investigated drug classes were antibiotics (10%), antipsychotics (9%), antidiabetics (8%), anthelmintics (6%), and antihistamines (6%). Frequently studied drugs included niclosamide, metformin, atorvastatin, and doxazosin, targeting pathways such as PI3K/AKT/mTOR, apoptosis, and autophagy. Bibliometric analysis revealed increasing scientific output, with emphasis on combination therapies, cellular mechanisms, and technologies like molecular docking and nanosystems. These findings highlight the growing relevance of drug repurposing in lung cancer, especially in accelerating effective therapy discovery using approved compounds. Progress in this field depends on integrating diverse methodologies and fostering interdisciplinary collaboration. As a next step, rigorous clinical trials are essential to confirm the efficacy and safety of promising repurposed agents in oncology.
Aspergillus spp. in woodworking settings: implications for occupational health and safety
Publication . Dias, Marta; Rodriguez, Margarida; Riesenberger, Bruna; Marques, Liliana; Carolino, Elisabete; Twarużek, Magdalena; Kosicki, Robert; Gonçalves, Lídia; Caetano, Liliana Aranha; Alastruey-Izquierdo, Ana; Viegas, Susana; Viegas, Carla
Woodworkers are exposed to several potentially harmful agents, including microorganisms that grow in the wood. The most common fungal species in woodworking environments are Aspergillus, Penicillium, and Cladosporium spp., with occupational exposure to Aspergillus spp. posing a significant respiratory risk. This study aimed to assess exposure to Aspergillus spp. in two Portuguese woodworking environments and to perform a thorough analysis of Aspergillus fumigatus complex isolates collected from 13 DIY stores and 6 Carpentries in the Lisbon Metropolitan Area. Sampling combined active and passive methods to assess microbial contamination. Aspergillus fumigatus isolates were analysed for their antifungal susceptibility, resistant mechanisms, mycotoxin production, and cytotoxic potential in lung (A459) and liver (HepG2) cell lines. The MAS-100 presented Aspergillus sections Aspergilli and Flavi with the highest prevalence in DIY stores and Carpentries, respectively. A total of 1185 Aspergillus spp. were recovered, 270 identified as Aspergillus fumigatus sensu stricto growing at 37 °C. None of those isolates was resistant to azoles, 99.07% of them produced gliotoxin, and 39.9% of them produced cytotoxic effects in at least one cell line. This study comprehended a multi-approach that considered not only sampling methods but also the laboratory assays to be applied in the Aspergillus section Fumigati isolates recovered from two different woodworking environments, allowing a complete and robust analysis of this specific environment and species. Overall, the findings indicate that woodworkers are exposed to A. fumigatus isolates with relevant pathogenic traits, despite the absence of azole resistance, underscoring the need for continued environmental and occupational monitoring.
The indoor microbiome: sampling, analysis and emerging trends
Publication . Šunić, Iva; Šarac, Jelena; Auguštin, Dubravka Havaš; Pozdniakova, Sofya; Ferguson, Robert M.; Jergović, Matijana; Visentin, David; Borràs, Silvia; Archer, Elizabeth; Henderson, Drew K.; Vitko, Sandra; Ašić, Adna; Bošnjaković, Anja; Maglica, Željka; Viegas, Carla; Novokmet, Natalija; Karlović, Nina; Marjanović, Damir; Muszyński, Adam; Liu, Yuxi; Karisola, Piia; Alenius, Harri; Krych, Lukasz; Lovrić, Mario
Indoor spaces contain diverse microbial communities that shape human health. These microorganisms are particularly relevant to respiratory diseases, including asthma and allergies. Despite growing recognition of the importance of indoor microbial exposures, research in this field is slowed by differences in methods. These inconsistencies make it difficult to compare results and draw conclusions. This systematic review analyses 106 studies published between 2000 and 2025 that investigated indoor microbiomes in dust, air, and other matrices across homes, schools, and other built environments. We assessed sampling strategies, DNA extraction protocols, sequencing technologies, and bioinformatic pipelines, identifying trends, inconsistencies, and areas requiring harmonisation. Passive sampling, particularly dust collection, was the most common approach, while Illumina-based 16S rRNA and ITS amplicon sequencing dominated molecular analyses. However, variations in targeted gene regions, extraction kits, and analytical tools limited cross-study comparability. Ecological findings revealed consistent detection of bacterial taxa such as Staphylococcus, Streptococcus, and Corynebacterium, and fungal taxa including Cladosporium, Aspergillus, and Penicillium, with diversity shaped by building characteristics, ventilation, humidity, occupancy, and presence of pets. This review highlights the need for standardised protocols in indoor microbiome research to facilitate reproducibility, enable meta-analyses, and inform health-related guidelines for indoor environments.
Política sobre Acesso Aberto a publicações científicas resultantes de investigação financiada pela FCT
Publication . Antunes, Maria Luz; Lopes, Carlos
A Fundação para a Ciência e a Tecnologia (FCT) lançou a sua nova Política sobre Acesso Aberto a Publicações Científicas resultantes de investigação financiada pela FCT, que se traduz numa revisão e atualização significativas dos mecanismos em vigor desde 2014. A revisão da anterior Política resulta da experiência da sua aplicação ao longo dos últimos 10 anos, dos desenvolvimentos muito significativos verificados no tratamento do acesso aberto por instituições das quais Portugal faz parte, tais como a UNESCO ou a União Europeia (UE), e também da crescente consciência dos benefícios da publicação em acesso aberto, como forma de disseminar o acesso ao conhecimento. Os contornos desta revisão são também orientados pela adesão da FCT ao Plano S, promovido pela cOAlition S e apoiado pela Science Europe. [sic FCT]