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Advisor(s)
Abstract(s)
Antimicrobial resistance of human pathogens, such as methicillin-resistant Staphylococcus aureus, is described by the World Health Organization as a health global challenge and efforts must be made for the discovery of new effective and safe compounds. This work aims to evaluate epigallocatechin-3-gallate (EGCG) epigenetic and modulatory drug potential against S. aureus in vitro and in vivo. S. aureus strains were isolated from the commensal flora of healthy volunteers. Antibiotic susceptibility and synergistic assay were assessed through disk diffusion accordingly to EUCAST guidelines with and without co-exposure to EGCG at final concentrations of 250 µg/ml, 100 µg/ml, 50 µg/ml, and 25 µg/ml. Transcriptional expression of orfx, spdC, and WalKR was performed through qRT-PCR. A 90-day interventional study was performed with daily consumption of 225 mg of EGCG. Obtained data revealed a high prevalence of S. aureus colonization in healthcare workers and clearly demonstrated the antimicrobial and synergistic potential of EGCG as well as divergent resistant phenotypes associated with altered transcriptional expression of epigenetic and drug response modulators genes. Here, we demonstrate the potential of EGCG for antimicrobial treatment and/or therapeutic adjuvant against antibiotic-resistant microorganisms and report divergent patterns of epigenetic modulators expression associated with phenotypic resistance profiles.
Description
Project “Resistance modulation and epigenetic divergence in resistant phenotypic profiles of Staphylococcus aureus” (IPL/2020/ EpiResistanceSA _ESTeSL).
FCT_UIDB/05608/2020. FCT_UIDP/05608/2020.
FCT_UIDB/05608/2020. FCT_UIDP/05608/2020.
Keywords
Anti-bacterial agents Anti-infective agents Catechin Drug resistance Epigenesis, Genetic Methicillin-resistant Staphylococcus aureus Microbial sensitivity tests Staphylococcal Infections Staphylococcal aureus Project Resistance modulation and epigenetic divergence in resistant phenotypic profiles of Staphylococcus aureus IPL/2020/ EpiResistanceSA _ESTeSL FCT_UIDB/05608/2020 FCT_UIDP/05608/2020
Citation
Zeferino AS, Mira AR, Delgadinho M, Brito M, Ponte T, Ribeiro E. Drug resistance and epigenetic modulatory potential of epigallocatechin-3-gallate against Staphylococcus aureus. Curr Microbiol. 2022;79(5):149.