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Drug resistance and epigenetic modulatory potential of epigallocatechin-3-gallate against Staphylococcus aureus

2022-04Journal article Open access
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dc.contributor.authorZeferino, Ana Sofia
dc.contributor.authorMira, Ana Rita
dc.contributor.authorDelgadinho, Mariana
dc.contributor.authorBrito, Miguel
dc.contributor.authorPonte, Tomás
dc.contributor.authorRibeiro, Edna
dc.date.accessioned2022-04-18T16:50:21Z
dc.date.available2024-04-19T00:30:22Z
dc.date.issued2022-04
dc.descriptionProject “Resistance modulation and epigenetic divergence in resistant phenotypic profiles of Staphylococcus aureus” (IPL/2020/ EpiResistanceSA _ESTeSL).pt_PT
dc.descriptionFCT_UIDB/05608/2020. FCT_UIDP/05608/2020.pt_PT
dc.description.abstractAntimicrobial resistance of human pathogens, such as methicillin-resistant Staphylococcus aureus, is described by the World Health Organization as a health global challenge and efforts must be made for the discovery of new effective and safe compounds. This work aims to evaluate epigallocatechin-3-gallate (EGCG) epigenetic and modulatory drug potential against S. aureus in vitro and in vivo. S. aureus strains were isolated from the commensal flora of healthy volunteers. Antibiotic susceptibility and synergistic assay were assessed through disk diffusion accordingly to EUCAST guidelines with and without co-exposure to EGCG at final concentrations of 250 µg/ml, 100 µg/ml, 50 µg/ml, and 25 µg/ml. Transcriptional expression of orfx, spdC, and WalKR was performed through qRT-PCR. A 90-day interventional study was performed with daily consumption of 225 mg of EGCG. Obtained data revealed a high prevalence of S. aureus colonization in healthcare workers and clearly demonstrated the antimicrobial and synergistic potential of EGCG as well as divergent resistant phenotypes associated with altered transcriptional expression of epigenetic and drug response modulators genes. Here, we demonstrate the potential of EGCG for antimicrobial treatment and/or therapeutic adjuvant against antibiotic-resistant microorganisms and report divergent patterns of epigenetic modulators expression associated with phenotypic resistance profiles.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationZeferino AS, Mira AR, Delgadinho M, Brito M, Ponte T, Ribeiro E. Drug resistance and epigenetic modulatory potential of epigallocatechin-3-gallate against Staphylococcus aureus. Curr Microbiol. 2022;79(5):149.pt_PT
dc.identifier.doi10.1007/s00284-022-02841-5pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/14566
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationProject “Resistance modulation and epigenetic divergence in resistant phenotypic profiles of Staphylococcus aureus” (IPL/2020/ EpiResistanceSA _ESTeSL)pt_PT
dc.relationFCT_UIDB/05608/2020pt_PT
dc.relationFCT_UIDP/05608/2020pt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s00284-022-02841-5pt_PT
dc.subjectAnti-bacterial agentspt_PT
dc.subjectAnti-infective agentspt_PT
dc.subjectCatechinpt_PT
dc.subjectDrug resistancept_PT
dc.subjectEpigenesis, Geneticpt_PT
dc.subjectMethicillin-resistant Staphylococcus aureuspt_PT
dc.subjectMicrobial sensitivity testspt_PT
dc.subjectStaphylococcal Infectionspt_PT
dc.subjectStaphylococcal aureuspt_PT
dc.subjectProject Resistance modulation and epigenetic divergence in resistant phenotypic profiles of Staphylococcus aureuspt_PT
dc.subjectIPL/2020/ EpiResistanceSA _ESTeSLpt_PT
dc.subjectFCT_UIDB/05608/2020pt_PT
dc.subjectFCT_UIDP/05608/2020pt_PT
dc.titleDrug resistance and epigenetic modulatory potential of epigallocatechin-3-gallate against Staphylococcus aureuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue5pt_PT
oaire.citation.startPage149pt_PT
oaire.citation.titleCurrent Microbiologypt_PT
oaire.citation.volume79pt_PT
person.familyNameBrito
person.familyNameRibeiro
person.givenNameMiguel
person.givenNameEdna
person.identifier.ciencia-id231F-F341-7E93
person.identifier.ciencia-idC414-CDF2-D35A
person.identifier.orcid0000-0001-6394-658X
person.identifier.orcid0000-0003-1316-7750
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id35224551000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
relation.isAuthorOfPublicationa571bf34-bcda-49ca-b5cb-4cdecbb3d9c7
relation.isAuthorOfPublication.latestForDiscoverya571bf34-bcda-49ca-b5cb-4cdecbb3d9c7

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