| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| Abstract | 105.65 KB | Adobe PDF | ||
| Poster | 2.94 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Epigallocatechin-3-gallate (EGCG) antioxidant properties have been demonstrated however, increasing evidence indicates that EGCG produces reactive oxygen species. The aim of this study was to evaluate the effect of EGCG intake during 90 days in hematological cardiovascular risk factors, vitamins A and E, DNA damage, and oxidative damage in human blood. Peripheral blood from 30 healthy individuals (10 males and 20 females; 18 – 45 years), was collected at time 0 (T0) and time 90 (T90). During 90 days, participants ingested capsules of green tea extract (225mg EGCG) daily. Hematological cardiovascular risk factors including lipid profile and liver function parameters were assessed using colorimetric methods. Vitamins A and E in serum were quantified by HPLC and analysis of DNA damage and oxidative damage was performed by comet assay. Our results showed that lipid profile and liver function parameters are not affected by EGCG and serum levels of vitamin E increased, but not vitamin A. An increase in DNA damage and DNA oxidative damage after 90 days of EGCG consumption was also reported. The results suggest that EGCG can induce DNA damage, possibly due to ROS induction, with an associated increase of the antioxidant vitamin E, however without alteration of hematological cardiovascular risk factors.
Description
Keywords
Epigallocatechin-3-gallate Green tea Oxidative stress DNA damage
Citation
Ladeira C, Pádua M, Ramos C, Ribeiro E. Green tea epigallocatechin-3-gallate (EGCG) oxidative stress and DNA damage. In: SFRRE Annual Meeting Abstracts - Society for Free Radical Research Europe, “Redox Homeostasis: From Signaling to Damage”, Ferrara (Italy), June 19-21, 2019. Free Radical Biol Med. 2019;139 Suppl 1:S33.
Publisher
Elsevier