Distribution of CCR5-Delta32, CCR2-64I, and SDF1-3’A host genetic factors in HIV-infected and uninfected individuals in Luanda, Angola

Background: The HIV/AIDS pandemic remains a public health concern. Studies on host genetic polymorphisms that confer resistance to HIV-1 infection or delay HIV disease progression are scarce in African countries. Herein, we investigate the proportion of the mutated phenotype of the AIDS-related polymorphisms CCR5-Delta32, CCR2-64I, and SDF1-3'A in HIV-infected and uninfected individuals in Luanda, the capital of Angola, a sub-Saharan African country. Methods: This was a cross-sectional study conducted with 284 individuals, of whom 159 were HIV-negative and 125 were HIV-positive. The CCR5-Delta32, CCR2-64I, and SDF1-3'A genotypes were detected by conventional PCR and visualised on a 2% agarose gel. A Chi-square test determined the frequency of each genetic variant and was deemed significant when p < 0.05. Results: The frequency of CCR5-Delta32, CCR2-64I, and SDF1-3 A was 0% (0/272), 60.2% (154/256), and 42.5% (114/268), respectively. CCR2-64I and SDF1-3 A polymorphisms were statistically related to HIV infection (p < 0.001). Statistically significant was observed between ABO blood groups (p = 0.006) and HIV-1 subtype (p = 0.015) with CCR2-64I. Also, the age group (p = 0.024) and RH blood group (p = 0.018) were statistically related to the distribution of SDF1-3 A polymorphism. Conclusions: We found no CCR5-Delta32 allele, while CCR2-64I and SDF1-3'A were found and presented a relationship with HIV infection, age, ABO/RH blood group, and HIV-1 subtypes. The observed associations of CCR2-64I and SDF1-3'A with HIV underscore the urgent need for further multidisciplinary research, with potential implications for targeted prevention and public health strategies. Therefore, studies investigating biological and non-biological factors related to susceptibility to HIV infection and AIDS progression or death should be conducted in Angola.

Description

This research was funded by the Fundação Calouste Gulbenkian (FCG), under the ENVOLVE Ciência PALOP program that funded the HITOLA project (Number 250466), FCT MARVEL (PTDC/SAU-PUB/4018/2021), FCT GHTMUID/04413/2020, and LA-REAL-LA/P/0117/2020.

Keywords

AIDS-related gene variants Allelic frequency HIV infection Angola Luanda

URI

http://hdl.handle.net/10400.21/21907

Citation

Sebastião CS, Pimentel V, Jandondo D, Sebastião JM, Sacomboio E, Brito M, et al. Distribution of CCR5-Delta32, CCR2-64I, and SDF1-3'A host genetic factors in HIV-infected and uninfected individuals in Luanda, Angola. AIDS Res Ther. 2025;22(1):54.