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Advisor(s)
Abstract(s)
A new method to simultaneously predict cells
viability, proliferation and metabolic status, in a rapid, simple but
also specific and sensitive mode was developed. The method is based
on mid-infrared (MIR) spectroscopic analysis of cells. As model
system were used Human embryonic kidney (HEK) 293 cells and T lymphocytes. After submitting cells to different environments as the
toxic dimethyl sulfoxide, or metabolic activation, cells viability was
analyzed by optical microscopy after coloration with trypan blue, and
the cell count was determined with a Neubauer hemocytometer. The
principal component analysis (PCA) of the cells second derivative
spectra enabled to discriminate the cells viability and the cells
proliferation as assayed by conventional methods, while spectra PCA and Hierarchical Cluster Analysis (HCA) enabled to discriminate T cells metabolic activation. The new methods, based on MIR spectroscopy, present the advantages of being applicable in automatic, simple and high-throughput mode in relation to the onventional methods.
Description
Keywords
Cells viability Cells proliferation FTIR spectroscopy
Pedagogical Context
Citation
V. Caldeira. R. Araújo, L. Ramalhete, CRC Calado. Predict cells viability, proliferation, and metabolic status, based in one unique and simple assay. In: 1st International Conference on Challenges in Engineering, Medical, Economics and Education: Research & Solutions (CEMEERS-23). [International Forum of Engineers and Practitioners, June 21-22, 2023, ISEL, Lisbon, Portugal].
Publisher
https://fenp.org/procceeding.php/39