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Predict cells viability, proliferation, and metabolic status, based in one unique and simple assay

2023Conference object Open access
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dc.contributor.authorCaldeira, Viviana
dc.contributor.authorAraújo, Rúben
dc.contributor.authorRamalhete, Luís
dc.contributor.authorCalado, Cecília
dc.date.accessioned2024-03-22T17:11:36Z
dc.date.available2024-03-22T17:11:36Z
dc.date.issued2023
dc.description.abstractA new method to simultaneously predict cells viability, proliferation and metabolic status, in a rapid, simple but also specific and sensitive mode was developed. The method is based on mid-infrared (MIR) spectroscopic analysis of cells. As model system were used Human embryonic kidney (HEK) 293 cells and T lymphocytes. After submitting cells to different environments as the toxic dimethyl sulfoxide, or metabolic activation, cells viability was analyzed by optical microscopy after coloration with trypan blue, and the cell count was determined with a Neubauer hemocytometer. The principal component analysis (PCA) of the cells second derivative spectra enabled to discriminate the cells viability and the cells proliferation as assayed by conventional methods, while spectra PCA and Hierarchical Cluster Analysis (HCA) enabled to discriminate T cells metabolic activation. The new methods, based on MIR spectroscopy, present the advantages of being applicable in automatic, simple and high-throughput mode in relation to the onventional methods.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationV. Caldeira. R. Araújo, L. Ramalhete, CRC Calado. Predict cells viability, proliferation, and metabolic status, based in one unique and simple assay. In: 1st International Conference on Challenges in Engineering, Medical, Economics and Education: Research & Solutions (CEMEERS-23). [International Forum of Engineers and Practitioners, June 21-22, 2023, ISEL, Lisbon, Portugal].pt_PT
dc.identifier.doittps://doi.org/10.17758/EIRAI19.F0623104pt_PT
dc.identifier.isbn978-989-9121-26-3
dc.identifier.urihttp://hdl.handle.net/10400.21/17204
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherhttps://fenp.org/procceeding.php/39pt_PT
dc.relation.publisherversionhttps://fenp.org/procceeding.php/39pt_PT
dc.subjectCells viabilitypt_PT
dc.subjectCells proliferationpt_PT
dc.subjectFTIR spectroscopypt_PT
dc.titlePredict cells viability, proliferation, and metabolic status, based in one unique and simple assaypt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/DSAIPA%2FDS%2F0117%2F2020/PT
oaire.citation.conferencePlacePortugalpt_PT
oaire.citation.endPage2pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.title1st International Conference on Challenges in Engineering, Medical, Economics and Education: Research & Solutions (CEMEERS-23). [International Forum of Engineers and Practitionerspt_PT
oaire.fundingStream3599-PPCDT
person.familyNameModesto Caldeira
person.familyNameAraújo
person.familyNameRamalhete
person.familyNameCalado
person.givenNameViviana
person.givenNameRúben Alexandre Dinis
person.givenNameLuís
person.givenNameCecília
person.identifier2296066
person.identifier130332
person.identifier.ciencia-idBC11-91E8-1C16
person.identifier.ciencia-id9A18-BFDC-ED95
person.identifier.ciencia-idDF19-022D-AA10
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-1105-056X
person.identifier.orcid0000-0002-9369-6486
person.identifier.orcid0000-0002-8911-3380
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id57208672678
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublication9998e940-5e65-4661-8308-afcb56d5df01
relation.isAuthorOfPublication7846e088-851e-47b6-a7bd-d02c9d8f047d
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