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Integrating classical culture and molecular qPCR for a comprehensive assessment of fungal exposure in primary schools: preliminary data
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Orientador(es)
Resumo(s)
Children are a particularly vulnerable population to indoor fungal exposure. School environments can harbour complex and dynamic microbiomes containing allergenic, toxigenic, and pathogenic fungi. Characterisation often relies on a single method. This may underestimate true exposure. Some species also carry azole resistance, a growing clinical concern. Dual approaches capture viable and non-cultivable fractions. Objective: To compare culture and qPCR for assessing fungal exposure in primary schools.
Descrição
The authors gratefully acknowledge FCT/MCTES UIDP/05608/2020 and UIDB/05608/2020. This work is also supported by national funds through FCT/MCTES/FSE/UE, 2023.01366.BD, and 2025.02613.BD. This project was funded by the European Union (grant agreement: 101056883). Views and opinions expressed are, however, those of the author(s) only and do not necessarily reflect those of the European Health and Digital Executive Agency (HaDEA). Neither the European Union nor the granting authority can be held responsible for them. InChildHealth is also receiving funding from the Swiss State Secretariat for Education, Research and Innovation (SERI grant agreement 22.00324), from the United Kingdom Research and Innovation (UKRI grant agreement 10040524), and from the Australian National Health & Medical Research Council (NHMRC grant agreements APP2017786 and APP2008813).
Palavras-chave
Occupational health Occupational exposure Fungal exposure Primary school FCT_UIDP/05608/2020 FCT_UIDB/05608/2020
Contexto Educativo
Citação
Cervantes R, Pena P, Viegas C. Integrating classical culture and molecular qPCR for a comprehensive assessment of fungal exposure in primary schools: preliminary data. In: OHSI/ISES Europe 2026 Workshop – Prevention is better than the cure, University College of Dublin (Ireland), 10-11 June, 2026.