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Disruption and phenotypic analysis of six open reading frames from chromosome VII of Saccharomyces cerevisiae reveals one essential gene

2001-06Journal article Open access
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dc.contributor.authorGuerreiro, Paulo
dc.contributor.authorRodrigues-Pousada, Claudina
dc.date.accessioned2014-01-15T12:25:10Z
dc.date.available2014-01-15T12:25:10Z
dc.date.issued2001-06
dc.description.abstractSix open reading frames (ORFs) located on chromosome VII of Saccharomyces cerevisiae (YGR205w, YGR210c, YGR211w, YGR241c, YGR243w and YGR244c) were disrupted in two different genetic backgrounds using short-flanking homology (SFH) gene replacement. Sporulation and tetrad analysis showed that YGR211w, recently identified as the yeast ZPR1 gene, is an essential gene. The other five genes are non-essential, and no phenotypes could be associated to their inactivation. Two of these genes have recently been further characterized: YGR241c (YAP1802) encodes a yeast adaptor protein and YGR244c (LSC2) encodes the b-subunit of the succinyl-CoA ligase. For each ORF, a replacement cassette with long flanking regions homologous to the target locus was cloned in pUG7, and the cognate wild-type gene was cloned in pRS416.por
dc.identifier.citationGuerreiro P, Rodrigues-Pousada C. Disruption and phenotypic analysis of six open reading frames from chromosome VII of Saccharomyces cerevisiae reveals one essential gene. Yeast. 2001;18(9):781-7.por
dc.identifier.issn1097-0061
dc.identifier.urihttp://hdl.handle.net/10400.21/3086
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/yea.723/abstractpor
dc.subjectSaccharomyces cerevisiaepor
dc.subjectEUROFANpor
dc.subjectGene disruptionpor
dc.subjectZPR1por
dc.subjectYAP1802por
dc.subjectLSC2por
dc.subjectYHR162wpor
dc.subjectChromosomespor
dc.subjectGene deletionpor
dc.subjectMutagenesis, Insertionalpor
dc.subjectOpen reading frames/geneticspor
dc.subjectPhenotypepor
dc.subjectPlasmids/geneticspor
dc.subjectPolymerase chain reactionpor
dc.subjectTransformation, Geneticpor
dc.titleDisruption and phenotypic analysis of six open reading frames from chromosome VII of Saccharomyces cerevisiae reveals one essential genepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage787por
oaire.citation.startPage781por
oaire.citation.titleYeastpor
oaire.citation.volume18por
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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