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Orientador(es)
Resumo(s)
Six open reading frames (ORFs) located on chromosome VII of Saccharomyces cerevisiae
(YGR205w, YGR210c, YGR211w, YGR241c, YGR243w and YGR244c) were disrupted in two different genetic backgrounds using short-flanking homology (SFH) gene replacement. Sporulation and tetrad analysis showed that YGR211w, recently identified as the yeast ZPR1 gene, is an essential gene. The other five genes are non-essential, and no phenotypes could be associated to their inactivation. Two of these genes have recently been further characterized: YGR241c (YAP1802) encodes a yeast adaptor protein and YGR244c (LSC2) encodes the b-subunit of the succinyl-CoA ligase. For each ORF, a replacement cassette with long flanking regions homologous to the target locus was cloned in pUG7, and the cognate wild-type gene was cloned in pRS416.
Descrição
Palavras-chave
Saccharomyces cerevisiae EUROFAN Gene disruption ZPR1 YAP1802 LSC2 YHR162w Chromosomes Gene deletion Mutagenesis, Insertional Open reading frames/genetics Phenotype Plasmids/genetics Polymerase chain reaction Transformation, Genetic
Contexto Educativo
Citação
Guerreiro P, Rodrigues-Pousada C. Disruption and phenotypic analysis of six open reading frames from chromosome VII of Saccharomyces cerevisiae reveals one essential gene. Yeast. 2001;18(9):781-7.