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Balancing act: tubulin glutamylation and microtubule dynamics in Toxoplasma gondii

2024-01Preprint Open access
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dc.contributor.authorDelgado, Inês L.S.
dc.contributor.authorGonçalves, João
dc.contributor.authorFernandes, Rita
dc.contributor.authorZúquete, Sara
dc.contributor.authorBasto, Afonso P.
dc.contributor.authorLeitão, Alexandre
dc.contributor.authorSoares, Helena
dc.contributor.authorNolasco, Sofia
dc.date.accessioned2024-02-12T13:08:27Z
dc.date.available2024-02-12T13:08:27Z
dc.date.issued2024-01
dc.description.abstractThe success of Toxoplasma gondii (intracellular parasite) host cell invasion relies on the apical complex, a specialized microtubule cytoskeleton structure associated with secretory organelles. The genome encodes three isoforms of both α- and β-tubulin which are altered by specific post-translational modifications (PTMs), changing the biochemical/biophysical proprieties of microtubules, and modulating their interaction with associated proteins. Tubulin PTMs are a powerful and evolutionarily conserved mechanism to generate tubulin diversity, forming a biochemical ‘tubulin code’ that can be ‘read’ by microtubule-interacting factors. The T. gondii tubulin PTMs are: α-tubulin acetylation, α-tubulin detyrosination, Δ5α-tubulin, Δ2α-tubulin, α- and β-tubulin polyglutamylation, and α- and α-tubulin methylation. Tubulin glutamylation is a key candidate to assist microtubule remodeling in Toxoplasma, being involved in the regulation of microtubule stability, dynamics, interaction with motor proteins, and severing enzymes. The correct balance of tubulin glutamylation is achieved by the coordinated action of polyglutamylases and deglutamylating enzymes. In this work, we will review and discuss the current knowledge on T. gondii tubulin glutamylation. By in silico identification of mammalian protein orthologs, we explored and updated the identification of putative proteins related to glutamylation, contributing to a better understanding of the role of tubulin glutamylation in T. gondii.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDelgado IL, Gonçalves J, Fernandes R, Zúquete S, Soares H, Nolasco S, et al. Balancing act: tubulin glutamylation and microtubule dynamics in Toxoplasma gondii. Preprints.org. 2024:2024010287.pt_PT
dc.identifier.doi10.20944/preprints202401.0287.v1pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/17104
dc.language.isoengpt_PT
dc.peerreviewednopt_PT
dc.publisherPreprints.orgpt_PT
dc.relation.publisherversionhttps://www.preprints.org/manuscript/202401.0287/v1pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectToxoplasma gondiipt_PT
dc.subjectTubulin glutamylationpt_PT
dc.subjectTubulin post-translational modificationspt_PT
dc.subjectApical complexpt_PT
dc.subjectMicrotubulespt_PT
dc.titleBalancing act: tubulin glutamylation and microtubule dynamics in Toxoplasma gondiipt_PT
dc.typepreprint
dspace.entity.typePublication
oaire.citation.startPage2024010287pt_PT
person.familyNameAntunes Soares
person.givenNameMaria Helena
person.identifierG-3065-2010
person.identifier.ciencia-id131B-F0E1-572C
person.identifier.orcid0000-0001-6180-7041
person.identifier.scopus-author-id55932139400
rcaap.rightsopenAccesspt_PT
rcaap.typepreprintpt_PT
relation.isAuthorOfPublication267fae06-39c1-4b12-a246-39e0b1dde34a
relation.isAuthorOfPublication.latestForDiscovery267fae06-39c1-4b12-a246-39e0b1dde34a

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