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Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence

2020-12Journal article Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorDias, Marta
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorSabino, Raquel
dc.date.accessioned2020-12-29T15:20:27Z
dc.date.available2020-12-29T15:20:27Z
dc.date.issued2020-12
dc.descriptionProject EXPOsE. Project nº 23222 (02/SAICT/2016).pt_PT
dc.descriptionProject Waste FRPD. Project IPL/2018/WasteFRPD_ESTeSL.pt_PT
dc.descriptionProject Occupational exposure of ambulance drivers to bioburden. Project IPL/2020/BIO-AmbuDrivers_ESTeSL.pt_PT
dc.descriptionFCT_UIDB/05608/2020. FCT_UIDP/05608/2020.pt_PT
dc.description.abstractCulturing is still the most widely used method for determining fungal growth. Thus, is important to identify the most suitable culture media to assess Aspergillus spp. The aim of this study was to analyze data obtained from previous studies, aiming at identifying the most suitable culture media (malt extract agar (MEA) or dichloran-glycerol agar (DG18) to assess Aspergillus spp. isolation and growth. This study was conducted by using environmental samples (n = 1153). Most of the active sampling methods (air samples) were impacted directly onto both culture media. As for passive sampling methods, fungi were extracted from environmental matrices inoculated onto both media. Overall, total Aspergillus counts were higher in MEA (n = 617, 53.5%) than in DG18 (n = 536, 46.5%). Regarding Aspergillus sections, significant associations were detected with the media (χ2 (7) = 241.118, p < 0.001), the sampling approach (p < 0.001, 95% CI = (0.3 × 10−4), and the indoor environment (p < 0.001, 95% CI = (0.3 × 10−4)). As such, the sampling approach and the culture media should be accurately selected when dealing with Aspergillus spp. exposure assessment.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Dias M, Carolino E, Sabino R. Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence. Atmosphere. 2021;12:23.pt_PT
dc.identifier.doi10.3390/atmos12010023pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/12532
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationProject EXPOsEpt_PT
dc.relationProject nº 23222 (02/SAICT/2016)pt_PT
dc.relationProject Waste FRPDpt_PT
dc.relationProject IPL/2018/WasteFRPD_ESTeSLpt_PT
dc.relationProject Occupational exposure of ambulance drivers to bioburdenpt_PT
dc.relationProject IPL/2020/BIO-AmbuDrivers_ESTeSLpt_PT
dc.relationFCT_UIDB/05608/2020pt_PT
dc.relationFCT_UIDP/05608/2020pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2073-4433/12/1/23pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectAssessment exposurept_PT
dc.subjectMalt extract agarpt_PT
dc.subjectDG18pt_PT
dc.subjectSamplingpt_PT
dc.subjectIndoorpt_PT
dc.subjectAspergillus spppt_PT
dc.subjectProject EXPOsEpt_PT
dc.subjectProject nº 23222 (02/SAICT/2016)pt_PT
dc.subjectProject Waste FRPDpt_PT
dc.subjectIPL/2018/WasteFRPD_ESTeSLpt_PT
dc.subjectProject Occupational exposure of ambulance drivers to bioburdenpt_PT
dc.subjectIPL/2020/BIO-AmbuDrivers_ESTeSLpt_PT
dc.subjectFCT_UIDB/05608/2020pt_PT
dc.subjectFCT_UIDP/05608/2020pt_PT
dc.titleCulture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidencept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage23pt_PT
oaire.citation.titleAtmospherept_PT
oaire.citation.volume12pt_PT
person.familyNameViegas
person.familyNameCarolino
person.givenNameCarla
person.givenNameElisabete
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-4165-7052
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication.latestForDiscoveryb5fa5da4-50c3-4b88-ae20-1bc63cb485f7

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