Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence

Viegas, Carla; Dias, Marta; Carolino, Elisabete; Sabino, Raquel

http://hdl.handle.net/10400.21/12532

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Abstract(s)

Culturing is still the most widely used method for determining fungal growth. Thus, is important to identify the most suitable culture media to assess Aspergillus spp. The aim of this study was to analyze data obtained from previous studies, aiming at identifying the most suitable culture media (malt extract agar (MEA) or dichloran-glycerol agar (DG18) to assess Aspergillus spp. isolation and growth. This study was conducted by using environmental samples (n = 1153). Most of the active sampling methods (air samples) were impacted directly onto both culture media. As for passive sampling methods, fungi were extracted from environmental matrices inoculated onto both media. Overall, total Aspergillus counts were higher in MEA (n = 617, 53.5%) than in DG18 (n = 536, 46.5%). Regarding Aspergillus sections, significant associations were detected with the media (χ2 (7) = 241.118, p < 0.001), the sampling approach (p < 0.001, 95% CI = (0.3 × 10−4), and the indoor environment (p < 0.001, 95% CI = (0.3 × 10−4)). As such, the sampling approach and the culture media should be accurately selected when dealing with Aspergillus spp. exposure assessment.

Description

Project EXPOsE. Project nº 23222 (02/SAICT/2016).
Project Waste FRPD. Project IPL/2018/WasteFRPD_ESTeSL.
Project Occupational exposure of ambulance drivers to bioburden. Project IPL/2020/BIO-AmbuDrivers_ESTeSL.
FCT_UIDB/05608/2020. FCT_UIDP/05608/2020.

Keywords

Occupational health Occupational exposure Assessment exposure Malt extract agar DG18 Sampling Indoor Aspergillus spp Project EXPOsE Project nº 23222 (02/SAICT/2016) Project Waste FRPD IPL/2018/WasteFRPD_ESTeSL Project Occupational exposure of ambulance drivers to bioburden IPL/2020/BIO-AmbuDrivers_ESTeSL FCT_UIDB/05608/2020 FCT_UIDP/05608/2020

URI

http://hdl.handle.net/10400.21/12532

Citation

Viegas C, Dias M, Carolino E, Sabino R. Culture media and sampling collection method for Aspergillus spp. assessment: tackling the gap between recommendations and the scientific evidence. Atmosphere. 2021;12:23.