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- New pharmacological approach for inflammatory bowel diseasePublication . Silva, Inês; Pinto, Rui; Mateus, VanessaInflammatory bowel disease includes Crohn's disease and ulcerative colitis. Chronic inflammatory disease of the gastrointestinal (GI) tract is characterized by recurrent ulceration. IBD affects 7–10% of people worldwide, mainly of Caucasian descent. It manifests into several intestinal and extra-intestinal symptoms, mainly related to oxidative stress, inflammation, and autoimmune reaction. Aim of the study: evaluate the influence of a new pharmacological approach with erythropoietin in the establishment and development of inflammation associated with IBD, through of an experimental colitis model in rodents.
- TNBS-induced colitis in rodents: preliminary results of a chronic modelPublication . Silva, Inês; Mateus, Vanessa; Pinto, RuiBackground: Inflammatory bowel disease (IBD) is a gastrointestinal disorder characterized by chronic inflammation of the intestinal epithelium. The symptoms of IBD depend on the intestinal affected segment and usually include diarrhea often with blood, colic abdominal pain, and fecal urgency. Beyond these, other unspecific symptoms may occur like fever, loss of appetite and weight, fatigue, and primary amenorrhea. Nowadays, used therapy in IBD consists of salicylates, corticosteroids, immunosuppressants, and biological therapy. These drugs aim to induce and/or maintain the patient in remission and ameliorate the disease’s secondary effects, rather than modifying or reversing the underlying pathogenic mechanism. Aim: Development of an animal model of trinitrobenzene sulfonic acid (TNBS)-induced chronic colitis in order to evaluate the influence of new drugs in the IBD.
- TNBS-induced colitis in rodents: preliminary results of a chronic modelPublication . Silva, Inês; Solas, João; Sousa, Mariana; Modesto, Rita; Mateus, Vanessa; Pinto, RuiChronic inflammatory disease of the gastrointestinal (GI) tract characterized by recurrent ulceration. Treatment of inflammatory bowel disease induce and maintain the patient in remission However do not modify or reverse the underlying pathogenic mechanism. Nonclinical studies for emerging therapeutic strategies. Aim of the study: Evaluate the efficacy of new drugs in inflammatory bowel disease through an animal model of TNBS-induced chronic colitis.
- Preclinical study in vivo for new pharmacological approaches in inflammatory bowel disease: a systematic review of chronic model of TNBS-induced colitisPublication . Silva, Inês; Pinto, Rui; Mateus, VanessaThe preclinical studies in vivo provide means of characterizing physiologic interactions when our understanding of such processes is insufficient to allow replacement with in vitro systems and play a pivotal role in the development of a novel therapeutic drug cure. Chemically induced colitis models are relatively easy and rapid to develop. The 2,4,6-trinitrobenzenesulfonic acid (TNBS) colitis model is one of the main models in the experimental studies of inflammatory bowel disease (IBD) since inflammation induced by TNBS mimics several features of Crohn’s disease. This review aims to summarize the existing literature and discuss different protocols for the induction of chronic model of TNBS-induced colitis. We searched MEDLINE via Pubmed platform for studies published through December 2018, using MeSH terms (Crohn Disease.kw) OR (Inflammatory Bowel Diseases.kw) OR (Colitis, Ulcerative.kw) AND (trinitrobenzenesulfonic acid.kw) AND (disease models, animal.kw) AND (mice.all). The inclusion criteria were original articles, preclinical studies in vivo using mice, a chronic model of colitis, and TNBS as the inducer of colitis and articles published in English. Chronic TNBS-induced colitis is made with multiple TNBS intrarectal administrations in an average dose of 1.2 mg using a volume lower than 150 μL in 50% ethanol. The strains mostly used are Balb/c and C57BL/6 with 5–6 weeks. To characterize the preclinical model the parameters more used include body weight, stool consistency and morbidity, inflammatory biomarkers like interferon (IFN)-γ, myeloperoxidase (MPO), tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10, presence of ulcers, thickness or hyperemia in the colon, and histological evaluation of the inflammation. Experimental chronic colitis is induced by multiple rectal instillations of TNBS increasing doses in ethanol using Balb/c and C57BL/6 mice.