Browsing by Author "Oliveira, Filipe"
Now showing 1 - 4 of 4
Results Per Page
Sort Options
- Coffee production: an occupational setting where exposure to mycotoxins need to be recognized and preventedPublication . Viegas, Susana; Twaruzek, Magdalena; Kosicki, Robert; Oliveira, Filipe; Andrade, Enilton Tavares de; Viegas, CarlaCoffee production involves different processes until being available as a beverage involving several workers worldwide. Mycotoxin exposure may occur in this occupational setting since coffee beans are frequently contaminated with these fungal secondary metabolites due the crop infection by toxigenic fungi that commonly infect the plant during the various production stages. A study was developed in two milling industries located in Brazil aiming to evaluate workplace contamination with mycotoxins.
- Fungal and mycotoxins contamination in non-alcoholic beverages: the case of tea from Portuguese market and coffee beans from BrazilPublication . Viegas, Carla; Gomes, Bianca; Cervantes, Renata; Dias, Marta; Pena, Pedro; Oliveira, Filipe; Andrade, Ednilton Tavares de; Caetano, Liliana Aranha; Twaruzek, Magdalena; Kosicki, Robert; Viegas, SusanaAgriculture constitutes one of the most sensitive sectors that could be affected by climate change. Among the xenobiotics contaminating agricultural crops, fungi and mycotoxins are the most challenging since their presence represents an economic burden due to crop loss and serious health effects related to animals and humans with severe repercussions. This study intends to characterize the fungal and mycotoxin contamination in tea available in the Portuguese market and in coffee from two Brazilian industries.
- Microbial contamination in the coffee industry: an occupational menace besides a food safety concern?Publication . Viegas, Carla; Gomes, Bianca; Oliveira, Filipe; Dias, Marta; Cervantes, Renata; Pena, Pedro; Gomes, Anita Q.; Caetano, Liliana Aranha; Carolino, Elisabete; de Andrade, Ednilton Tavares; Viegas, SusanaRespiratory abnormalities among workers at coffee roasting and packaging facilities have already been reported; however, little is known about microbiological contamination inside coffee production facilities. This study intends to assess the microbial contamination (fungi and bacteria) in two coffee industries in Brazil with a multi-approach protocol for sampling and for subsequent analyses using four main sources of samples: filtering respiratory protection devices (FRPD) used by workers, settled dust, electrostatic dust cloths (EDC) and coffee beans. The fungal contamination in the assessed industries was also characterized through the molecular detection of toxigenic species and antifungal resistance. Total bacteria contamination presented the highest values in FRPD collected from both industries (7.45 × 104 CFU.m−2; 1.09 × 104 CFU.m−2). Aspergillus genera were widespread in all the environmental samples collected and sections with clinical relevance (Fumigati) and with toxigenic potential (Nigri and Circumdati) were recovered from FRPD. Circumdati section was observed in 4 mg/mL itraconazole. Sections Circumdati (EDC, coffee beans, and settled dust) and Nidulantes (EDC, coffee beans, and FRPD) were detected by qPCR. Some of the targeted Aspergillus sections that have been identified microscopically were not detected by qPCR and vice-versa. Overall, this study revealed that microbial contamination is a potential occupational risk in the milling stage and should be tackled when assessing exposure and performing a risk assessment. In addition, a multi-sampling campaign should be the approach to follow when assessing microbial contamination and FRPD should be included in this campaign. Occupational exposure to mycotoxins should be considered due to high fungal diversity and contamination. A One Health approach should address these issues in order to prevent the consumption of coffee crops and beans infected by fungi and, more specifically, to avoid widespread azole resistance.
- Mycotoxins contamination in Brazilian coffee industry: the need of a One Health ApproachPublication . Viegas, Susana; Oliveira, Filipe; Twaruzek, Magdalena; Kosicki, Robert; Andrade, Enilton Tavares de; Viegas, CarlaObjective: This work aims to characterize mycotoxin contamination in two coffee industries from Brazil using as sampling methods settled dust and coffee beans. Methods: A total of 58 samples from the two coffee industries were assessed (20 settled dust, 20 filters used to vacuum the settled dust, and 18 coffee beans) and screened for mycotoxins presence. In all samples, 38 mycotoxins were analyzed by HPL-MS ((HPLC) Nexera (Shimadzu, Tokyo, Japan) with a mass spectrometry detector API 4000 (Sciex, Foster City, CA, USA). The mycotoxin concentration was calculated using external calibration. Results: In settled dust, all the samples showed contamination by at least one mycotoxin being the most reported mycophenolic acid and ochratoxin A. In addition, more three mycotoxins were detected namely: fumonisin B1, ochratoxin B, and zearalenone. Three (15%) samples showed contamination by three different mycotoxins and twelve (60%) by two mycotoxins. In the coffee beans samples, four mycotoxins were detected (aflatoxin B2, fumonisin B2, ochratoxin A, and mycophenolic acid) being the most reported mycophenolic acid with 4 samples (20%) presenting contamination by two different mycotoxins. Conclusions: The observed mycotoxin contamination highlights the need to consider this occupational environment as critical regarding occupational exposure to this risk factor. In addition, it also pinpoints a potential menace concerning food safety since mycotoxins are not degraded in the coffee processing treatments. Future research should include a One Health approach to tackle the different risks in this setting.