Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/9121
Título: Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles
Autor: Ribeiro, A. P. C.
Anbu, S.
Alegria, Elisabete C. B. A.
Fernandes, A. R.
Baptista, P. V.
Mendes, R.
Matias, A. S.
Mendes, M.
Silva, M. F. C. Guedes da
Pombeiro, A. J. L.
Palavras-chave: Green chemistry
Silver nanoparticles
Cancer cell lines
Data: Mai-2018
Editora: Elsevier
Citação: RIBEIRO, A. P. C.; [et al] – Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles. Biomedicine & Pharmacotherapy. ISSN 0753-3322. Vol. 101, (2018), pp. 137-144
Resumo: Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 +/- 0.1 mu M) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 +/- 0.1 mu M). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.
Peer review: yes
URI: http://hdl.handle.net/10400.21/9121
DOI: 10.1016/j.biopha.2018.02.069
ISSN: 0753-3322
Versão do Editor: https://reader.elsevier.com/reader/sd/pii/S0753332217369500?token=1932B45DB896540BD5D45CC938D9ACE21A587526E82CDFA47D6DE3A13CD0C796D3035D719E282A33D836D6BF58621B36
Aparece nas colecções:ISEL - Eng. Quim. Biol. - Artigos

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