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Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles

2018-05Journal article Open access
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dc.contributor.authorDa Costa Ribeiro, Ana Paula
dc.contributor.authorAnbu, S
dc.contributor.authorAlegria, Elisabete
dc.contributor.authorFernandes, Alexandra
dc.contributor.authorBaptista, Pedro
dc.contributor.authorMendes, Rita
dc.contributor.authorMatias, A. S.
dc.contributor.authorMendes, M.
dc.contributor.authorGuedes Da Silva, M. Fátima C.
dc.contributor.authorPombeiro, Armando
dc.date.accessioned2018-12-03T12:02:02Z
dc.date.available2018-12-03T12:02:02Z
dc.date.issued2018-05
dc.description.abstractSilver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 +/- 0.1 mu M) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 +/- 0.1 mu M). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRIBEIRO, A. P. C.; [et al] – Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles. Biomedicine & Pharmacotherapy. ISSN 0753-3322. Vol. 101, (2018), pp. 137-144pt_PT
dc.identifier.doi10.1016/j.biopha.2018.02.069pt_PT
dc.identifier.issn0753-3322
dc.identifier.issn1950-6007
dc.identifier.urihttp://hdl.handle.net/10400.21/9121
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPOCI-01-0145-FEDER-007728 - ERDF under the PT2020 Partnership Agreementpt_PT
dc.relation.publisherversionhttps://reader.elsevier.com/reader/sd/pii/S0753332217369500?token=1932B45DB896540BD5D45CC938D9ACE21A587526E82CDFA47D6DE3A13CD0C796D3035D719E282A33D836D6BF58621B36pt_PT
dc.subjectGreen chemistrypt_PT
dc.subjectSilver nanoparticlespt_PT
dc.subjectInteractionpt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectCancer cell linespt_PT
dc.titleEvaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticlespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00100%2F2013/PT
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oaire.citation.endPage144pt_PT
oaire.citation.startPage137pt_PT
oaire.citation.titleBiomedicione & Pharmacotherapypt_PT
oaire.citation.volume101pt_PT
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person.familyNameda Costa Ribeiro
person.familyNameSellamuthu Kooduthurai
person.familyNameAlegria
person.familyNameFernandes
person.familyNameBaptista
person.familyNameSoares Mendes
person.familyNameGuedes da Silva
person.familyNamePombeiro
person.givenNameAna Paula
person.givenNameAnbu
person.givenNameElisabete
person.givenNameAlexandra
person.givenNamePedro
person.givenNameAna Rita
person.givenNameM. Fátima C.
person.givenNameArmando
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project.funder.nameFundação para a Ciência e a Tecnologia
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rcaap.typearticlept_PT
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