Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/8855
Título: Development of a flow injection analytical system for short chain amide determination based on a tubular bioreactor and an ammonium sensor
Autor: Veríssimo, Marta I. S.
Oliveira, Sara B.
Silva, Nelson A. F.
Matos, Manuel
Karmali, Amin
Gomes, M. Teresa S. R.
Palavras-chave: Pseudomonas aeruginosa
Biological membrane
Flow injection system
Ammonium acoustic wave sensor
Data: Ago-2018
Editora: Royal Society of Chemistry
Citação: VERÍSSIMO, Marta I. S.; [et al] – Development of a flow injection analytical system for short chain amide determination based on a tubular bioreactor and an ammonium sensor. Analyst. ISSN 0003-2654. Vol. 143, N.º 16 (2018), pp. 3859-3866
Resumo: Pseudomonas aeruginosa (P. aeruginosa) possesses intracellular amidase activity, which catalyses the hydrolysis of short aliphatic amides producing NH4 +, and has already been used along with an ammonium ion selective electrode for amide quantification. However, the incorporation of a biological membrane turned to be a challenging process and either the final arrangement was prone to amidase losses or the recovery of the sensor coating after the interaction took too long. In this article a flow injection system with an ammonium acoustic wave sensor is proposed, and after testing several different arrangements for the biological element, the ultimate choice consisted of the immobilization of a P. aeruginosa cell-free extract in the inner wall of a tubular glass reactor, which resulted in a reliable analytical system. Response times less than one minute and complete recovery in less than two minutes assured conveniently fast analysis. The analytical system, as long as the column was properly stored in HEPES buffer containing 2 mM β-mercaptoethanol and 1 mM benzamidine and refrigerated when not in use, could be used at least for 20 working days, along a period of one month, maintaining the initial sensitivity.
Peer review: yes
URI: http://hdl.handle.net/10400.21/8855
DOI: 10.1039/c8an00699g
ISSN: 0003-2654
1364-5528
Versão do Editor: https://pubs.rsc.org/en/content/articlepdf/2018/an/c8an00699g
Aparece nas colecções:ISEL - Eng. Quim. Biol. - Artigos

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