Repository logo
 
Publication

Development of a flow injection analytical system for short chain amide determination based on a tubular bioreactor and an ammonium sensor

2018-08-21Journal article Restricted access
Simple item page
dc.contributor.authorVeríssimo, M.I.S.
dc.contributor.authorOliveira, Sara B.
dc.contributor.authorSilva, Nelson A. F.
dc.contributor.authorMatos, Manuel
dc.contributor.authorKarmali, Amin
dc.contributor.authorGomes, Maria Teresa SR
dc.date.accessioned2018-09-11T10:40:26Z
dc.date.available2018-09-11T10:40:26Z
dc.date.issued2018-08-21
dc.description.abstractPseudomonas aeruginosa (P. aeruginosa) possesses intracellular amidase activity, which catalyses the hydrolysis of short aliphatic amides producing NH4 +, and has already been used along with an ammonium ion selective electrode for amide quantification. However, the incorporation of a biological membrane turned to be a challenging process and either the final arrangement was prone to amidase losses or the recovery of the sensor coating after the interaction took too long. In this article a flow injection system with an ammonium acoustic wave sensor is proposed, and after testing several different arrangements for the biological element, the ultimate choice consisted of the immobilization of a P. aeruginosa cell-free extract in the inner wall of a tubular glass reactor, which resulted in a reliable analytical system. Response times less than one minute and complete recovery in less than two minutes assured conveniently fast analysis. The analytical system, as long as the column was properly stored in HEPES buffer containing 2 mM β-mercaptoethanol and 1 mM benzamidine and refrigerated when not in use, could be used at least for 20 working days, along a period of one month, maintaining the initial sensitivity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVERÍSSIMO, Marta I. S.; [et al] – Development of a flow injection analytical system for short chain amide determination based on a tubular bioreactor and an ammonium sensor. Analyst. ISSN 0003-2654. Vol. 143, N.º 16 (2018), pp. 3859-3866pt_PT
dc.identifier.doi10.1039/c8an00699gpt_PT
dc.identifier.issn0003-2654
dc.identifier.issn1364-5528
dc.identifier.urihttp://hdl.handle.net/10400.21/8855
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationDEVELOPMENT OF AN OPTICAL FIBRE-SENSOR FOR VOC DETECTION IN CULTURAL HERITAGE APPLICATIONS
dc.relation.publisherversionhttps://pubs.rsc.org/en/content/articlepdf/2018/an/c8an00699gpt_PT
dc.subjectPseudomonas aeruginosapt_PT
dc.subjectBiological membranept_PT
dc.subjectFlow injection systempt_PT
dc.subjectAmmonium acoustic wave sensorpt_PT
dc.titleDevelopment of a flow injection analytical system for short chain amide determination based on a tubular bioreactor and an ammonium sensorpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDEVELOPMENT OF AN OPTICAL FIBRE-SENSOR FOR VOC DETECTION IN CULTURAL HERITAGE APPLICATIONS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAMB%2F50017%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F94267%2F2013/PT
oaire.citation.endPage3866pt_PT
oaire.citation.issue16pt_PT
oaire.citation.startPage3859pt_PT
oaire.citation.titleAnalystpt_PT
oaire.citation.volume143pt_PT
oaire.fundingStream5876
oaire.fundingStreamFARH
person.familyNameVeríssimo
person.familyNameSilva
person.familyNameMatos
person.familyNameKarmali
person.familyNameSeabra dos Reis Gomes
person.givenNameMarta
person.givenNameNelson Alberto Frade
person.givenNameManuel
person.givenNameAmin
person.givenNameMaria Teresa
person.identifierB-8317-2014
person.identifierC-2259-2012
person.identifier.ciencia-idEB12-17F7-642E
person.identifier.ciencia-id5F12-4509-C41E
person.identifier.ciencia-id2E14-6146-CC46
person.identifier.ciencia-id2A14-622F-2E4D
person.identifier.orcid0000-0003-3718-4985
person.identifier.orcid0000-0001-8646-1227
person.identifier.orcid0000-0002-5240-8070
person.identifier.orcid0000-0003-0419-401X
person.identifier.orcid0000-0003-3240-2883
person.identifier.ridE-4787-2011
person.identifier.ridC-1168-2013
person.identifier.scopus-author-id6603424315
person.identifier.scopus-author-id35588347400
person.identifier.scopus-author-id6603778216
person.identifier.scopus-author-id8084222000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbc922f54-ed04-4af6-a20c-6b4ea1048d95
relation.isAuthorOfPublicationeb1da4d4-5048-481a-b08b-c5dc05b9620c
relation.isAuthorOfPublicationbb104304-4226-4d6d-babf-a872d11e783b
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublication31c024b1-000b-418b-b95c-a76ad079f5f1
relation.isAuthorOfPublication.latestForDiscoverybb104304-4226-4d6d-babf-a872d11e783b
relation.isProjectOfPublication6b231382-2ec4-46c9-a9b5-51699bd7cab5
relation.isProjectOfPublication9ac06087-4695-4045-82d1-113de0c78307
relation.isProjectOfPublication.latestForDiscovery6b231382-2ec4-46c9-a9b5-51699bd7cab5

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Development_MMatos_ADEQ.pdf
Size:
1.66 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEL - Eng. Quim. Biol. - Artigos