Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/456
Título: Evaluation of staining techniques on the results of micronucleus in exfoliated oral mucosa cells
Autor: Mendonça, Paula
Ladeira, Carina
Palavras-chave: Anatomia patológica
Mucosa bucal
Data: Mai-2011
Citação: Mendonça P, Ladeira C. Evaluation of staining techniques on the results of micronucleus in exfoliated oral mucosa cells. In 35th European Congress of Cytology, Lisbon, 27th to 30th September 2011. Poster.
Resumo: Micronuclei (MN) in exfoliated epithelial cells are widely used as biomarkers of cancer risk in humans. MN are classified as biomarkers of the break age and loss of chromosomes. They are small, extra nuclear bodies that arise in dividing cells from centric chromosome/chromatid fragments or whole chromosomes/chromatids that lag behind in anaphase and are not included in the daughter nuclei in telophase. Buccal mucosa cells have been used in biomonitoring exposed populations because these cells are in the direct route of exposure to ingested pollutant, are capable of metabolizing proximate carcinogens to reactive chemicals, and are easily and rapidly collected by brushing the buccal mucosa. The objective of the present study was to further investigate if, and to what extent, different stains have an effect on the results of micronuclei studies in exfoliated cells. These techniques are: Papanicolaou (PAP), Modified Papanicolaou, May-Grünwald Giemsa (MGG), Giemsa, Harris’s Hematoxylin, Feulgen with Fast Green counterstain and Feulgen without counterstain.
Peer review: yes
URI: http://hdl.handle.net/10400.21/456
Aparece nas colecções:ESTeSL - Posters

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