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The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1

2023-07Preprint Open access
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dc.contributor.authorCarmona, Bruno
dc.contributor.authorCamelo, Carolina
dc.contributor.authorMehraz, Manon
dc.contributor.authorLemullois, Michel
dc.contributor.authorFaria, Mariana Lince
dc.contributor.authorCoyaud, Étienne
dc.contributor.authorMarinho, H. Susana
dc.contributor.authorGonçalves, João
dc.contributor.authorNolasco, Sofia
dc.contributor.authorPinto, Francisco
dc.contributor.authorRaught, Brian
dc.contributor.authorTassin, Anne-Marie
dc.contributor.authorKoll, France
dc.contributor.authorSoares, Helena
dc.date.accessioned2023-08-22T13:52:43Z
dc.date.available2023-08-22T13:52:43Z
dc.date.issued2023-07
dc.description.abstractCentrosomes are organelles consisting of two structurally and functionally distinct centrioles, with the mother centriole having complex distal (DA) and subdistal appendages (SDA). Despite their importance, how appendages are assembled and maintained remains unclear. This study investigated human TBCCD1, a centrosomal protein essential for centrosome positioning, to uncover its localization and role at centrioles. We found that TBCCD1 localizes at both proximal and distal regions of the two centrioles, forming a complex structure spanning from SDA to DA and extending inside and outside the centriole lumen. TBCCD1 depletion caused centrosome mispositioning, which was partially rescued by taxol, and the loss of microtubules (MTs) anchored to centrosomes. TBCCD1 depletion also reduced levels of SDA proteins involved in MT anchoring such as Centriolin/CEP110, Ninein, and CEP170. Additionally, TBCCD1 was essential for the correct positioning of motile cilia basal bodies and associated structures in Paramecium. This study reveals that TBCCD1 is an evolutionarily conserved protein essential for centriole and basal body localization and appendage assembly and maintenance. A BioID screening also linked TBCCD1 to ciliopathy-associated protein networks.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCarmona B, Camelo C, Mehraz M, Lemullois M, Nolasco S, Soares H, et al. The maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1. BioRxiv [preprint]. 2023 July 26.pt_PT
dc.identifier.doi10.1101/2023.07.26.549647pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/16386
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttps://www.biorxiv.org/content/10.1101/2023.07.26.549647v1pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectTBCCD1pt_PT
dc.subjectCentrosomept_PT
dc.subjectCentriole appendagespt_PT
dc.subjectCiliapt_PT
dc.subjectBasal bodypt_PT
dc.subjectMicrotubulespt_PT
dc.subjectParameciumpt_PT
dc.titleThe maintenance of centriole appendages and motile cilia basal body anchoring relies on TBCCD1pt_PT
dc.typepreprint
dspace.entity.typePublication
person.familyNameSousa Carmona
person.familyNameAntunes Soares
person.givenNameBruno Filipe
person.givenNameMaria Helena
person.identifierG-3065-2010
person.identifier.ciencia-id681F-6045-F8C2
person.identifier.ciencia-id131B-F0E1-572C
person.identifier.orcid0000-0003-0871-9063
person.identifier.orcid0000-0001-6180-7041
person.identifier.scopus-author-id55932139400
rcaap.rightsopenAccesspt_PT
rcaap.typepreprintpt_PT
relation.isAuthorOfPublication908e548e-eaac-4485-97c5-fcbd33fe7e5a
relation.isAuthorOfPublication267fae06-39c1-4b12-a246-39e0b1dde34a
relation.isAuthorOfPublication.latestForDiscovery267fae06-39c1-4b12-a246-39e0b1dde34a

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