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Analysis of surfaces for characterization of fungal burden: does it matter?

dc.contributor.authorViegas, Carla
dc.contributor.authorFaria, Tiago
dc.contributor.authorMeneses, Márcia
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorViegas, Susana
dc.contributor.authorGomes, Anita Quintal
dc.contributor.authorSabino, Raquel
dc.date.accessioned2016-06-29T15:18:53Z
dc.date.available2016-06-29T15:18:53Z
dc.date.issued2016
dc.description.abstractObjectives: Mycological contamination of occupational environments can be a result of fungal spores’ dispersion in the air and on surfaces. Therefore, it is very important to assess it in both types of the samples. In the present study we assessed fungal contamination in the air and in the surface samples to show relevance of surfaces sampling in complementing the results obtained in the air samples. Material and Methods: In total, 42 settings were assessed by the analysis of air and surfaces samples. The settings were divided into settings with a high fungal load (7 poultry farms and 7 pig farms, 3 cork industries, 3 waste management plants, 2 wastewater treatment plants and 1 horse stable) and a low fungal load (10 hospital canteens, 8 college canteens and 1 maternity hospital). In addition to culture-based methods, molecular tools were also applied to detect fungal burden in the settings with a higher fungal load. Results: From the 218 sampling sites, 140 (64.2%) presented different species in the examined surfaces when compared with the species identified in the air. A positive association in the high fungal load settings was found between the presence of different species in the air and surfaces. Wastewater treatment plants constituted the setting with the highest number of different species between the air and surface. Conclusions: We observed that surfaces sampling and application of molecular tools showed the same efficacy of species detection in high fungal load settings, corroborating the fact that surface sampling is crucial for a correct and complete analysis of occupational scenarios.pt_PT
dc.identifier.citationViegas C, Faria T, Meneses M, Carolino E, Viegas S, Gomes AQ, et al. Analysis of surfaces for characterization of fungal burden: does it matter? Int J Occup Med Environ Health. 2016;29(4):623-32.pt_PT
dc.identifier.doi10.13075/ijomeh.1896.00562pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/6259
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttp://ijomeh.eu/Analysis-of-surfaces-for-characterization-of-fungal-burden-Does-it-matter-,59242,0,2.htmlpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectOccupational environmentpt_PT
dc.subjectSurface samplespt_PT
dc.subjectFungal burden assessmentpt_PT
dc.subjectHigh fungal load settingspt_PT
dc.subjectLow fungal load settingspt_PT
dc.subjectAir samplespt_PT
dc.subjectFungal contaminationpt_PT
dc.subjectMycologypt_PT
dc.titleAnalysis of surfaces for characterization of fungal burden: does it matter?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage632
oaire.citation.issue4pt_PT
oaire.citation.startPage623
oaire.citation.titleInternational Journal of Occupational Medicine and Environmental Healthpt_PT
oaire.citation.volume29pt_PT
person.familyNameViegas
person.familyNameCarolino
person.familyNameViegas
person.familyNameSabino
person.givenNameCarla
person.givenNameElisabete
person.givenNameSusana
person.givenNameRaquel Filipa Pinheiro
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1015-8760
person.identifier.orcid0000-0001-6585-7775
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35270591500
person.identifier.scopus-author-id35796067600
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublicationcf64684a-fb92-4421-91da-14fb15462fad
relation.isAuthorOfPublication.latestForDiscoverycf64684a-fb92-4421-91da-14fb15462fad

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