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Bioburden in health care centers: is the compliance with Portuguese legislation enough to prevent and control infection?

2019-08Journal article Restricted access
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dc.contributor.authorViegas, Carla
dc.contributor.authorAlmeida, Beatriz
dc.contributor.authorMonteiro, Ana
dc.contributor.authorAranha Caetano, Liliana
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorGomes, Anita Quintal
dc.contributor.authorTwarużek, Magdalena
dc.contributor.authorKosicki, Robert
dc.contributor.authorMarchand, Geneviéve
dc.contributor.authorViegas, Susana
dc.date.accessioned2019-06-26T11:17:40Z
dc.date.available2019-06-26T11:17:40Z
dc.date.issued2019-08
dc.descriptionEXPOsE – Establishing protocols to assess occupational exposure to microbiota in clinical settings. Project nº 23222 (02/SAICT/2016).pt_PT
dc.description.abstractThis study intends to assess the bioburden in ten Portuguese health care centers (PHCC) with a multi-approach protocol using active (air sampling by impaction and impinger) and passive (surface swabs and air-conditioning filters) sampling methods, and compare the results with compliance levels of IAQ Portuguese legislation for the assessment of bioburden. The fungal burden in the PHCC was also characterized through molecular detection of toxigenic species, antifungal resistance and mycotoxins profile. Concerning legal compliance criteria for bacteriota the first criteria [indoor] + 350 CFU. m−3 < [outdoor] was not compiled in all PHCC analyzed. Regarding fungal load, 60% did not comply with the quantitative guideline (I/O < 1). Growth of four fungal genera (Chrysosporium, Cladosporium, Mucor, and Penicillium) was observed in HVAC filter samples collected from 6 out of 10 assessed PHCC when using azole-supplemented SDA media. Overall, Aspergillus/Penicillium/Paecilomyces levels were above the detection limits, except in one PHCC. The presence of mycotoxins was found both in air and HVAC filter samples. From the air samples analyzed, nine were contaminated (ng/ml) with 1–5 different mycotoxins within the same sample, whereas in HVAC filters, four samples contained 1 or 2 mycotoxins in the same filter. Overall, it was possible to conclude that Portuguese legislation is not enough to ensure IAQ in health care settings. The multi-approach sampling protocol used in this study allowed to unveil a more real scenario regarding exposure to bioburden. Detection of mycotoxins reinforces the relevance of studying mycotoxins in the clinical environment.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Monteiro A, Caetano LA, Carolino E, Gomes AQ, Viegas S, et al. Bioburden in health care centers: Is the compliance with Portuguese legislation enough to prevent and control infection? Build Environ. 2019;160:106226.pt_PT
dc.identifier.doi10.1016/j.buildenv.2019.106226pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/10209
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationEXPOsE – Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/SAICT/2016 – Project nº 23222)pt_PT
dc.relationProject nº 23222 (02/SAICT/2016)pt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0360132319304366pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectPublic healthpt_PT
dc.subjectEnvironmental exposurept_PT
dc.subjectHealth care centerpt_PT
dc.subjectMulti-approach sampling protocolpt_PT
dc.subjectAspergilluspt_PT
dc.subjectAzole resistancept_PT
dc.subjectMycotoxinspt_PT
dc.subjectProject EXPOsEpt_PT
dc.subjectProject nº 23222 (02/SAICT/2016)pt_PT
dc.titleBioburden in health care centers: is the compliance with Portuguese legislation enough to prevent and control infection?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage106226pt_PT
oaire.citation.titleBuilding and Environmentpt_PT
oaire.citation.volume160pt_PT
person.familyNameViegas
person.familyNameAranha Caetano
person.familyNameCarolino
person.familyNameViegas
person.givenNameCarla
person.givenNameLiliana
person.givenNameElisabete
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id9716-9DAC-532A
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-1496-2609
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35270591500
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication6517c656-f913-4f54-8682-77c2856c9e4c
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscovery6517c656-f913-4f54-8682-77c2856c9e4c

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