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OsRMC, a negative regulator of salt stress response in rice, is regulated by two AP2/ERF transcription factors

2013-07Journal article Open access
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dc.contributor.authorSerra, Tânia S.
dc.contributor.authorFigueiredo, Duarte D.
dc.contributor.authorCordeiro, André M.
dc.contributor.authorAlmeida, Diego M.
dc.contributor.authorLourenço, Tiago
dc.contributor.authorAbreu, Isabel A.
dc.contributor.authorSebastián, Alvaro
dc.contributor.authorFernandes, Lisete
dc.contributor.authorContreras-Moreira, Bruno
dc.contributor.authorOliveira, M. Margarida
dc.contributor.authorSaibo, Nelson J.
dc.date.accessioned2013-12-31T18:37:14Z
dc.date.available2013-12-31T18:37:14Z
dc.date.issued2013-07
dc.description.abstractHigh salinity causes remarkable losses in rice productivity worldwide mainly because it inhibits growth and reduces grain yield. To cope with environmental changes, plants evolved several adaptive mechanisms, which involve the regulation of many stress-responsive genes. Among these, we have chosen OsRMC to study its transcriptional regulation in rice seedlings subjected to high salinity. Its transcription was highly induced by salt treatment and showed a stress-dose-dependent pattern. OsRMC encodes a receptor-like kinase described as a negative regulator of salt stress responses in rice. To investigate how OsRMC is regulated in response to high salinity, a salt-induced rice cDNA expression library was constructed and subsequently screened using the yeast one-hybrid system and the OsRMC promoter as bait. Thereby, two transcription factors (TFs), OsEREBP1 and OsEREBP2, belonging to the AP2/ERF family were identified. Both TFs were shown to bind to the same GCC-like DNA motif in OsRMC promoter and to negatively regulate its gene expression. The identified TFs were characterized regarding their gene expression under different abiotic stress conditions. This study revealed that OsEREBP1 transcript level is not significantly affected by salt, ABA or severe cold (5 °C) and is only slightly regulated by drought and moderate cold. On the other hand, the OsEREBP2 transcript level increased after cold, ABA, drought and high salinity treatments, indicating that OsEREBP2 may play a central role mediating the response to different abiotic stresses. Gene expression analysis in rice varieties with contrasting salt tolerance further suggests that OsEREBP2 is involved in salt stress response in rice.por
dc.identifier.citationSerra TS, Figueiredo DD, Cordeiro AM, Almeida DM, Lourenço T, Fernandes L, et al. OsRMC, a negative regulator of salt stress response in rice, is regulated by two AP2/ERF transcription factors. Plant Mol Biol. 2013;82(4-5):439-55.por
dc.identifier.issn1573-5028
dc.identifier.urihttp://hdl.handle.net/10400.21/3036
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs11103-013-0073-9por
dc.subjectPlant sciencespor
dc.subjectBiochemistrypor
dc.subjectABApor
dc.subjectAbiotic stresspor
dc.subjectAdverse environmental conditionspor
dc.subjectHigh salinitypor
dc.subjectColdpor
dc.subjectDroughtpor
dc.subjectTranscriptional regulationpor
dc.subjectEREBPpor
dc.subjectYeast one-hybridpor
dc.subjectPhosphorylationpor
dc.subjectEMSApor
dc.titleOsRMC, a negative regulator of salt stress response in rice, is regulated by two AP2/ERF transcription factorspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage455por
oaire.citation.startPage439por
oaire.citation.titlePlant Molecular Biologypor
oaire.citation.volume82por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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