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Cytotoxicity effects of bisphenol A in Hep2 and MRC5 cell lines: is established TDI protective enough?

2017-07Conference object Open access
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dc.contributor.authorRibeiro, Edna
dc.contributor.authorZeferino, Ana Sofia
dc.contributor.authorDias, Ana
dc.contributor.authorCristovam, Elisabete
dc.contributor.authorViegas, Susana
dc.date.accessioned2018-12-30T18:42:52Z
dc.date.available2018-12-30T18:42:52Z
dc.date.issued2017-07
dc.description.abstractEndocrine disrupting chemicals (EDCs) are exogenous compounds generally employed in the food and drinks processing and packing, with potential associated hazardous effects for human health. Bisphenol A (BPA) stands out as a paradigmatic xenoestrogen for which human exposure is widespread, omnipresent and persistent, particularly trough ingestion of contaminated food and beverages. European Food Safety Authority (EFSA) experts on the latest comprehensive re-evaluation of BPA exposure and toxicity reduced the tolerable daily intake (TDI) from 50 μg/kg bw/day to 4 μg/kg bw/day. This compound is included in the Priority List of Chemicals within the EU Strategy for Endocrine Disruptors and classified as high concern in terms of human/wildlife exposure. BPA even at very low concentrations can influence cell fate in a cell type specific manner and epidemiological studies have demonstrated positive correlations between BPA levels detected in human biological samples and etiology of numerous pathologies. Here we aimed to evaluate potential cytotoxic effects of BPA concentrations within the range detected in human biological samples consistent to exposure levels below the established TDI, namely 4.4 μM, 4.4 nM and 0.44 nM in Hep2 and MRC5 cell lines. Cytotoxic effects were assessed trough the fluorometric method CellTiter-Blue assay and expression of BPA high affinity receptor GPER, in MRC5 cells, was performed trough immunofluorescence technique. We demonstrate that MCR5 cells express GPER receptor and no significant differences in viability associated with BPA exposure was observed in both cell lines. Our results indicate that BPA concentrations below the EFSA established TDI do not affect Hep2 or MRC5 cellular viability which sustains the indication of safe levels. Nevertheless, further research must be performed in order to assess potential effects that are not reflected by cytotoxicity such as genotoxicity and mixtures interactions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRibeiro E, Zeferino AS, Dias A, Cristovam E, Viegas S. Cytotoxicity effects of bisphenol A in Hep2 and MRC5 cell lines: is established TDI protective enough? In: Abrunhosa L, Venâncio A, Alvito P, editors. ICFC-2017 Book of abstracts – International Conference on Food Contaminants, Braga (Portugal), 13-14 July 2017. p. 85.pt_PT
dc.identifier.doi10.21814/icfc2017pt_PT
dc.identifier.isbn9789899747890
dc.identifier.urihttp://hdl.handle.net/10400.21/9225
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttps://repositorium.sdum.uminho.pt/bitstream/1822/46220/1/ICFC2017_Book_of_Abstracts_Web_2ndEd.pdfpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBisphenol Apt_PT
dc.subjectHuman exposurept_PT
dc.subjectCytotoxicity effectspt_PT
dc.titleCytotoxicity effects of bisphenol A in Hep2 and MRC5 cell lines: is established TDI protective enough?pt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceBragapt_PT
person.familyNameRibeiro
person.familyNameViegas
person.givenNameEdna
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idC414-CDF2-D35A
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0003-1316-7750
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id35270591500
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationa571bf34-bcda-49ca-b5cb-4cdecbb3d9c7
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscoverya571bf34-bcda-49ca-b5cb-4cdecbb3d9c7

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