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Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis

2022-04Journal article Open access
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dc.contributor.authorPimenta, Jorge M.
dc.contributor.authorPires, Virgínia M.
dc.contributor.authorNolasco, Sofia
dc.contributor.authorCastelo-Branco, Pedro
dc.contributor.authorMarques, Carla C.
dc.contributor.authorApolónio, Joana
dc.contributor.authorAzevedo, Rita
dc.contributor.authorFernandes, Mónica T.
dc.contributor.authorLopes-da-Costa, Luís
dc.contributor.authorPrates, José
dc.contributor.authorPereira, Rosa M.
dc.date.accessioned2022-02-11T10:45:38Z
dc.date.available2024-04-30T00:30:20Z
dc.date.issued2022-04
dc.descriptionFCT_UIDB/00276/2020pt_PT
dc.descriptionALT20-03-0246-FEDER-000021pt_PT
dc.description.abstractPrion proteins constitute a major public health concern, which has partly overshadowed their physiological roles in several scenarios. Indeed, these proteins were implicated in male fertility but their role in female fertility is relatively less explored. This study was designed to evaluate the role of SPRN and PRNP prion family genes in bovine follicular steroidogenesis pathways. Post-transcriptional SPRN and PRNP silencing with siRNAs was established in bovine granulosa cell (GC) in vitro culture, and gene expression and progesterone and estradiol concentrations were evaluated. SPRN knockdown, led to a downregulation of CYP11A1 mRNA levels (2.1-fold), and PRNP knockdown led to an upregulation of SPRN mRNA levels (2.3-fold). CYP19A1 expression and estradiol synthesis was not detected in any experimental group. Finally, SPRN knockdown led to a mild reduction in progesterone production in GCs and this was the only experimental group that did not exhibit an increment in progesterone levels after 48 h of culture. As a conclusion, it was possible to detect the expression of the SPRN gene in bovine GCs, a potential interaction between SPRN and PRNP regulation, and the impact of SPRN expression on CYP11A1 and progesterone levels. These findings bring new insights into the role of these genes in ovarian steroidogenesis and female reproductive physiology.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPimenta JM, Pires MR, Nolasco S, Castelo-Branco P, Marques CC, Apolónio J, et al. Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis. Biochem Biophys Res Commun. 2022;598:95-9.pt_PT
dc.identifier.doi10.1016/j.bbrc.2022.02.012pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/14284
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationFCT_UIDB/00276/2020pt_PT
dc.relationALT20-03-0246-FEDER-000021pt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0006291X22001942?via%3Dihubpt_PT
dc.subjectBovine granulosa cellspt_PT
dc.subjectPrion-like genespt_PT
dc.subjectsiRNApt_PT
dc.subjectSteroidogenesispt_PT
dc.subjectFCT_UIDB/00276/2020pt_PT
dc.subjectALT20-03-0246-FEDER-000021pt_PT
dc.titlePost-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage99pt_PT
oaire.citation.startPage95pt_PT
oaire.citation.titleBiochemical and Biophysical Research Communicationspt_PT
oaire.citation.volume598pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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