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The comet assay as a tool in human biomonitoring exposure to pesticides: a systematic review and meta-analysis

2025-10Journal article Embargoed access
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datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorGerić, Marko
dc.contributor.authorAzqueta, Amaya
dc.contributor.authorMøller, Peter
dc.contributor.authorBankoglu, Ezgi Eyluel
dc.contributor.authorGajski, Goran
dc.contributor.authorGiovannelli, Lisa
dc.contributor.authorHaverić, Anja
dc.contributor.authorStopper, Helga
dc.contributor.authorCollins, Andrew
dc.contributor.authorLadeira, Carina
dc.date.accessioned2025-12-22T09:14:03Z
dc.date.available2025-12-22T09:14:03Z
dc.date.issued2025-10
dc.descriptionThis work was supported by the affiliated institutions, European Regional Development Fund project KK.01.1.1.02.0007 (Rec-IMI), the European Union—Next Generation EU 533–03–23–0006 (BioMolTox), European Cooperation in Science and Technology (COST) Action 24108 - Capstone, and the International Comet Assay Working Group (ICAWG).
dc.description.abstractExposure to pesticides, most usually in occupational settings, is associated with different adverse health effects. In this systematic review and meta-analysis, we have assessed the effects of pesticide exposure on the level of DNA strand breaks in human peripheral blood cells, measured by the comet assay, in human biomonitoring studies. The literature search led to 80 studies included in the review. Of these, 66 studies met the criteria to be used in the meta-analysis. Using standardized mean difference and 95 % confidence interval (CI), the meta-analyses show an increased level of DNA strand breaks in subjects exposed to pesticides (2.02, 95 % CI: 1.69, 2.35). Results originate mainly from studies on workers, with only a few studies on environmental pesticide exposure. Subgroup analysis indicates that all studies combined from middle-income countries have a higher effect size (2.22, CI: 1.84, 2.59, n = 55) than studies from high-income countries (1.09, CI: 0.41, 1.76, n = 11). This difference between middle- and high-income countries may be mostly due to legislative, economic, and socio-cultural aspects. It has to be pointed out that only 9 % of the studies were classified as having an overall low risk of bias, while 12 % of the studies used exposure biomarkers. In conclusion, this systematic review and meta-analysis show that exposure to pesticides is associated with increased levels of DNA strand breaks in human peripheral blood cells.eng
dc.identifier.citationGerić M, Azqueta A, Møller P, Bankoglu EE, Gajski G, Ladeira C, et al. The comet assay as a tool in human biomonitoring exposure to pesticides: a systematic review and meta-analysis. Mutat Res Rev Mutat Res. 2025;796:108565.
dc.identifier.doi10.1016/j.mrrev.2025.108565
dc.identifier.issn1383-5742
dc.identifier.urihttp://hdl.handle.net/10400.21/22384
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier BV
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S1383574225000365
dc.relation.ispartofMutation Research - Reviews in Mutation Research
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectOccupational exposure
dc.subjectPesticides
dc.subjectPublic health
dc.subjectDNA damage
dc.subjectGenotoxicity
dc.titleThe comet assay as a tool in human biomonitoring exposure to pesticides: a systematic review and meta-analysiseng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage108565
oaire.citation.titleMutation Research - Reviews in Mutation Research
oaire.citation.volume796
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLadeira
person.givenNameCarina
person.identifier144237
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.orcid0000-0001-5588-0074
person.identifier.ridJ-2572-2012
person.identifier.scopus-author-id36463788000
relation.isAuthorOfPublication1aef4b60-4197-436b-84ab-80d31cbaed33
relation.isAuthorOfPublication.latestForDiscovery1aef4b60-4197-436b-84ab-80d31cbaed33

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