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Fungal contamination and azole-resistance in tea and other medicinal plants commercially available in Portugal

2021-09Conference object Open access
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dc.contributor.authorSimões, A.
dc.contributor.authorFaria, M.
dc.contributor.authorDias, M.
dc.contributor.authorViegas, Carla
dc.contributor.authorCaetano, Liliana Aranha
dc.date.accessioned2021-10-12T11:26:56Z
dc.date.available2021-10-12T11:26:56Z
dc.date.issued2021-09
dc.descriptionIPL/2020/TEAResMyc_ESTeSL. Project "Resistant mycobiota and mycotoxigenic profile of tea and medicinal plants”.pt_PT
dc.descriptionFCT_UIDB/05608/2020. FCT_UIDP/05608/2020.pt_PT
dc.description.abstractIntroduction. Tea and infusions are common beverages worldwide, prepared by infusing Camellia sinensis or other medicinal plants (eg. Melissa officinalis) with boiling water. Contamination by specific strains of filamentous fungi, such as Aspergillus sp., may originate health risks. While azole antifungal drugs are first-line therapy against fungal infections, the exposure of medicinal plants to azole fungicides in conventional agriculture can promote azole resistance, leading to therapeutic failure. Considering the growing risks to public health, the fungal contamination of tea and medicinal samples commercially available in Portugal was evaluated. Methodology. Samples (N=40) of different origins of conventional and biological agriculture were collected at supermarkets and herbalist shops in January 2021. Infusions were prepared according to ISO 3103:2019. Samples were also extracted as previously described and inoculated in fungal selective culture media (MEA and DG18) and in Sabouraud dextrose agar (SDA) medium supplemented with 4 mg/L itraconazole (ITR), 2 mg/L voriconazole (VOR), and 0.5 mg/L posaconazole (POS). Plates were incubated at optimal conditions for fungal growth. Fungal counts were determined as colony forming units (CFU) per gram and fungal morphology was analyzed by microscopy. Results. The average mycobiota was 359.1 ± 816.2 (MEA) and 559.1 ± 1263.8 (DG18) CFU/g in extracted samples, and 341.7 ± 277.2 (MEA) and 183.3 ± 271.6 (DG18) CFU/g in infusions. Regarding azole resistance, the most prevalent species were Aspergillus sp. (48% ITR) and C. sitophila (39% ITR, 47% VOR, 92% POS) in extracted samples, and C. sitophila (50% ITR, 37% VOR, 57% POS) in infusion samples. Among Aspergillus sp., the most prevalent sections were Nigri (96% MEA, 74% DG18, 72% SDA) and Circumdati (100% ITR) in extracted samples, and Fumigati (46% MEA, 100% SDA) in infusion. Conclusions. Fungal contaminants were present in tea samples and medicinal plants for infusion, including azole-resistant fungi. Relevance. The presence of Aspergillus sp., particularly section Fumigati, resistant to azoles in infusions may represent a health risk for consumers, especially immunocompromised individuals. Mycotoxins detection and correlational statistical analysis are ongoing to further determine whether there is a relation between sample origin, fungal contamination, and mycotoxigenic profile.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSimões A, Faria M, Dias M, Viegas C, Caetano LA. Fungal contamination and azole-resistance in tea and other medicinal plants commercially available in Portugal. In: International Conference on Food Contaminants – ICFC 2021 [online], Instituto Nacional de Saúde Dr. Ricardo Jorge (Portugal), September 27-28, 2021.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/13867
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationFCT_UIDB/05608/2020pt_PT
dc.relationFCT_UIDP/05608/2020pt_PT
dc.relationIPL/2020/TEAResMyc_ESTeSLpt_PT
dc.relation.publisherversionhttps://icfcportugal.com/wp-content/uploads/2021/09/ICFC-2021-Book-of-Abstracts.pdfpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAzole resistancept_PT
dc.subjectFungal contaminationpt_PT
dc.subjectTeapt_PT
dc.subjectMedicinal plantspt_PT
dc.subjectAspergillus fumigatuspt_PT
dc.subjectPortugalpt_PT
dc.subjectFCT_UIDB/05608/2020pt_PT
dc.subjectFCT_UIDP/05608/2020.pt_PT
dc.subjectIPL/2020/TEAResMyc_ESTeSLpt_PT
dc.titleFungal contamination and azole-resistance in tea and other medicinal plants commercially available in Portugalpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceLisboapt_PT
person.familyNameViegas
person.familyNameAranha Caetano
person.givenNameCarla
person.givenNameLiliana
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id9716-9DAC-532A
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-1496-2609
person.identifier.ridB-7217-2013
person.identifier.scopus-author-id55443609700
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication6517c656-f913-4f54-8682-77c2856c9e4c
relation.isAuthorOfPublication.latestForDiscoveryb5fa5da4-50c3-4b88-ae20-1bc63cb485f7

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