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The effects of waste sorting in environmental microbiome, THP-1 cell viability and inflammatory responses

2020-06Journal article Restricted access
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dc.contributor.authorViegas, Carla
dc.contributor.authorAranha Caetano, Liliana
dc.contributor.authorCox, J.
dc.contributor.authorKorkalainen, M.
dc.contributor.authorHaines, S.R.
dc.contributor.authorDannemiller, K.C.
dc.contributor.authorViegas, Susana
dc.contributor.authorReponen, T.
dc.date.accessioned2020-04-06T10:48:20Z
dc.date.available2020-04-06T10:48:20Z
dc.date.issued2020-06
dc.descriptionProject EXPOsE - Project nº 23222 (02/SAICT/2016). Project "Waste Workers' Exposure to Bioburden through Filtering Respiratory Protective Devices” - Project IPL/2018/WasteFRPD_ESTeSL.pt_PT
dc.description.abstractWorkers in the waste sorting industry are exposed to diverse bioaerosols. Characterization of these bioaerosols is necessary to more accurately assess the health risks of exposure. The use of high-throughput DNA sequencing for improved analysis of the microbial composition of bioaerosols, in combination with their in vitro study in relevant cell cultures, represents an important opportunity to find answers on the biological effects of bioaerosols. This study aimed to characterize by high-throughput sequencing the biodiversity present in complex aerosol mixtures retained in forklift air conditioning filters of a waste-sorting industry and its effects on cytotoxicity and secretion of proinflammatory cytokines in vitro using human macrophages derived from monocytic THP-1 cells. Seventeen filters from the filtration system from forklifts operating in one waste sorting facility and one control filter (similar filter without prior use) were analyzed using high-throughput sequencing and toxicological tests in vitro. A trend of positive correlation was seen between the number of bacterial and fungal OTUs (r = 0.47, p = 0.06). Seven filters (39%) exhibited low or moderate cytotoxicity (p < 0.05). The highest cytotoxic responses had a reduction in cell viability between 17 and 22%. Filter samples evoked proinflammatory responses, especially the production of TNFα. No significant correlation was found between fungal richness and inflammatory responses in vitro. The data obtained stress the need of thorough exposure assessment in waste-sorting industry and to take immunomodulatory properties into consideration for bioaerosols hazard characterization. The broad spectrum of microbial contamination detected in this study demonstrates that adequate monitoring of bioaerosol exposure is necessary to evaluate and minimize risks. The combined techniques can support the implementation of effective environmental monitoring programs of public and occupational health importance.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Caetano LA, Cox J, Korkalainen M, Haines SR, Viegas S, et al. The effects of waste sorting in environmental microbiome, THP-1 cell viability and inflammatory responses. Environ Res. 2020;185:109450.pt_PT
dc.identifier.doi10.1016/j.envres.2020.109450pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/11411
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationProject EXPOsEpt_PT
dc.relationProject nº 23222 (02/SAICT/2016)pt_PT
dc.relationProject Waste Workers' Exposure to Bioburden through Filtering Respiratory Protective Devicespt_PT
dc.relationProject IPL/2018/WasteFRPD_ESTeSLpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S0013935120303431pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectOccupational exposure assessmentpt_PT
dc.subjectWaste-sorting industrypt_PT
dc.subjectMicrobial diversitypt_PT
dc.subjectHigh-throughput sequencingpt_PT
dc.subjectCell viabilitypt_PT
dc.subjectProinflammatory cytokine levelspt_PT
dc.subjectProject EXPOsEpt_PT
dc.subjectProject nº 23222 (02/SAICT/2016)pt_PT
dc.subjectProject Waste Workers' Exposure to Bioburden through Filtering Respiratory Protective Devicespt_PT
dc.subjectProject IPL/2018/WasteFRPD_ESTeSLpt_PT
dc.titleThe effects of waste sorting in environmental microbiome, THP-1 cell viability and inflammatory responsespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage109450pt_PT
oaire.citation.titleEnvironmental Researchpt_PT
oaire.citation.volume185pt_PT
person.familyNameViegas
person.familyNameAranha Caetano
person.familyNameViegas
person.givenNameCarla
person.givenNameLiliana
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id9716-9DAC-532A
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-1496-2609
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id35270591500
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication6517c656-f913-4f54-8682-77c2856c9e4c
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscoveryb5fa5da4-50c3-4b88-ae20-1bc63cb485f7

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