Ecosapentanoic acid (EPA) does not affect cell kinetics in peripheral lymphocites from patients with Crohn's disease (CD) according to IL6 174G/C polymorphism

Brito, Miguel; Ferreira, Paula; Guerreiro, Catarina Sousa; Malta-Vacas, Joana; Tavares, L.; Santos, P.; Pinto, A.; Cravo, Marília

http://hdl.handle.net/10400.21/3364

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Authors

Brito, Miguel

Ferreira, Paula

Guerreiro, Catarina Sousa

Abstract(s)

Rationale: Omega 3 fatty acids have been shown to be of potential benefit in patients with CD. The aim of the present study was to evaluate whether EPA can modulate the inflammatory response according to different genotypes of IL6G174G/C polymorphism. Methods: Peripheral blood cells were collected from CD patients with different genotypes for IL6 174G/C (GG, n = 16, GC, n = 8, CC, n = 7), and lymphocytes were established in culture media. Replicates with the addition of EPA (25 mM) were analysed in a period of 24h, 48h and 72h. Expression of IL6 e a PGE2 was assessed by ELISA. Apoptosis and cellular proliferation was determined by flow cytometry.

Keywords

Crohn disease Ecosapentanoic acid Genotypes IL6 174 CC

URI

http://hdl.handle.net/10400.21/3364

Citation

Brito M, Ferreira P, Guerreiro CS, Malta-Vacas J, Tavares L, Santos P, et al. Ecosapentanoic acid (EPA) does not affect cell kinetics in peripheral lymphocites from patients with Crohn's disease (CD) according to IL6 174G/C polymorphism. Clin Nutr Suppl. 2008;3 Suppl 1:53.