Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles

Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 +/- 0.1 mu M) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 +/- 0.1 mu M). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.

Keywords

Green chemistry Silver nanoparticles Interaction Cytotoxicity Cancer cell lines

URI

http://hdl.handle.net/10400.21/9121

Citation

RIBEIRO, A. P. C.; [et al] – Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles. Biomedicine & Pharmacotherapy. ISSN 0753-3322. Vol. 101, (2018), pp. 137-144