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A new approach to assess occupational exposure to airborne fungal contamination and mycotoxins of forklift drivers in waste sorting facilities

2017-07Journal article Restricted access
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dc.contributor.authorViegas, Carla
dc.contributor.authorFaria, Tiago
dc.contributor.authorOliveira, Ana Cebola de
dc.contributor.authorCaetano, Liliana Aranha
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorGomes, Anita Quintalpt_PT
dc.contributor.authorTwarużek, Magdalenapt_PT
dc.contributor.authorKosicki, Robertpt_PT
dc.contributor.authorSoszczyńska, Ewelinapt_PT
dc.contributor.authorViegas, Susana
dc.date.accessioned2016-08-23T15:33:36Z
dc.date.available2016-08-23T15:33:36Z
dc.date.issued2017-07
dc.description.abstractThe waste management industry is an important employer, and exposure of waste-handling workers to microorganisms is considered an occupational health problem. Besides fungal contamination, it is important to consider the co-occurrence of mycotoxins in this setting. Forklifts with closed cabinet and air conditioner are commonly used in waste industry to transport waste and other products within the facilities, possibly increasing the risk of exposure under certain conditions. The aim of this study was to assess the fungal contamination and mycotoxin levels in filters from the air conditioning system of forklift cabinets, as an indicator to assess occupational exposure of the drivers working in a waste sorting facility. Cytotoxicity was also assessed to understand and characterize the toxicity of the complex mixtures as present in the forklift filters. Aqueous extracts of filters from 11 vehicles were streaked onto 2% malt extract agar (MEA) with chloramphenicol (0.05 g/L) media, and in dichloran glycerol (DG18) agar-based media for morphological identification of the mycobiota. Real-time quantitative PCR amplification of genes from Aspergillus sections Fumigati, Flavi, Circumdati, and Versicolores was also performed. Mycotoxins were analyzed using LC-MS/MS system. Cytotoxicity of filter extracts was analyzed by using a MTT cell culture test. Aspergillus species were found most frequently, namely Aspergillus sections Circumdati (MEA 48%; DG18 41%) and Nigri (MEA 32%; DG18 17.3%). By qPCR, only Aspergillus section Fumigati species were found, but positive results were obtained for all assessed filters. No mycotoxins were detected in aqueous filter extracts, but most extracts were highly cytotoxic (n = 6) or medium cytotoxic (n = 4). Although filter service life and cytotoxicity were not clearly correlated, the results suggest that observing air conditioner filter replacement frequency may be a critical aspect to avoid worker's exposure. Further research is required to check if the environmental conditions as present in the filters could allow the production of mycotoxins and their dissemination in the cabinet during the normal use of the vehicles.pt_PT
dc.identifier.citationViegas C, Faria T, de Oliveira AC, Caetano LA, Carolino E, Quintal-Gomes A, Viegas S, et al. A new approach to assess occupational exposure to airborne fungal contamination and mycotoxins of forklift drivers in waste sorting facilities. Mycotoxin Res. 2017 Jul 20. [Epub ahead of print]pt_PT
dc.identifier.doi10.1007/s12550-017-0288-8pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/6398
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007%2Fs12550-017-0288-8pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectOccupational exposurept_PT
dc.subjectFungal contaminationpt_PT
dc.subjectCytotoxicity evaluationpt_PT
dc.subjectFilters from forklift cabinetpt_PT
dc.subjectMycotoxinspt_PT
dc.titleA new approach to assess occupational exposure to airborne fungal contamination and mycotoxins of forklift drivers in waste sorting facilitiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage295pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage285pt_PT
oaire.citation.titleMycotoxin Researchpt_PT
oaire.citation.volume33pt_PT
person.familyNameViegas
person.familyNameCarolino
person.familyNameViegas
person.givenNameCarla
person.givenNameElisabete
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35270591500
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscoveryb5fa5da4-50c3-4b88-ae20-1bc63cb485f7

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