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Exposure assessment in one central hospital: a multi-approach protocol to achieve an accurate risk characterization

2020-02Journal article Restricted access
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dc.contributor.authorViegas, Carla
dc.contributor.authorAlmeida, Beatriz
dc.contributor.authorMonteiro, Ana
dc.contributor.authorPaciência, Inês
dc.contributor.authorRufo, João
dc.contributor.authorAguiar, Lívia
dc.contributor.authorLage, Bruna
dc.contributor.authorDiogo Gonçalves, Lídia Maria
dc.contributor.authorAranha Caetano, Liliana
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorGomes, Anita Quintal
dc.contributor.authorTwarużek, Magdalena
dc.contributor.authorKosicki, Robert
dc.contributor.authorGrajewski, Jan
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorViegas, Susana
dc.contributor.authorPereira, Cristiana
dc.date.accessioned2019-11-19T17:58:53Z
dc.date.available2019-11-19T17:58:53Z
dc.date.issued2020-02
dc.descriptionProject EXPOsE, project nº 23222 (02/SAICT/2016)pt_PT
dc.description.abstractThe bioburden in a Hospital building originates not only from patients, visitors and staff, but is also disseminated by several indoor hospital characteristics and outdoor environmental sources. This study intends to assess the exposure to bioburden in one central hospital with a multi-approach protocol using active and passive sampling methods. The microbial contamination was also characterized through molecular tools for toxigenic species, antifungal resistance and mycotoxins, and endotoxins profile. Two cytotoxicity assays (MTT and resazurin) were conducted with two cell lines (Calu-3 and THP-1), and in vitro pro-inflammatory potential was assessed in the THP-1 cell line. Out of the 15 sampling locations, 33.3% did not comply with Portuguese legislation regarding bacterial contamination, whereas concerning fungal contamination 60% presented I/O > 1. Toxigenic fungal species were observed in 27% of the sampled rooms (4 out of 15) and qPCR analysis successfully amplified DNA from the Aspergillus sections Flavi and Fumigati, although mycotoxins were not detected. Growth of distinct fungal species was observed on Sabouraud dextrose agar with triazole drugs, such as Aspergillus section Versicolores on 1 mg/L VORI. The highest concentrations of endotoxins were found in settled dust samples and ranged from 5.72 to 23.0 EU.mg−1. While a considerable cytotoxic effect (cell viability < 30%) was observed in one HVAC filter sample with the Calu-3 cell line, it was not observed with the THP-1 cell line. In air samples, a medium cytotoxic effect (61–68% cell viability) was observed in 3 out of 15 samples. The cytokine responses produced a more potent average cell response (46.8 ± 12.3 ρg/mL IL-1β; 90.8 ± 58.5 ρg/mL TNF-α) on passive samples than air samples (25.5 ± 5.2 ρg/mL IL-1β and of 19.4 ± 5.2 ρg/mL TNF-α). A multi-approach regarding parameters to assess, sampling, and analysis methods should be followed to characterize the bioburden in the hospital indoor environment. This study supports the importance of considering exposure to complex mixtures in indoor environments.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Almeida B, Monteiro A, Caetano LA, Carolino E, Gomes AQ, Viegas S, et al. Exposure assessment in one central hospital: a multi-approach protocol to achieve an accurate risk characterization. Environ Res. 2020;181:108947.pt_PT
dc.identifier.doi10.1016/j.envres.2019.108947pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/10717
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationProject EXPOsE, project nº 23222 (02/SAICT/2016)pt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0013935119307443pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectActive and passive sampling methodspt_PT
dc.subjectBioburdenpt_PT
dc.subjectAzole resistancept_PT
dc.subjectEndotoxinspt_PT
dc.subjectCytotoxicity assessmentpt_PT
dc.subjectHospitalpt_PT
dc.subjectPortugalpt_PT
dc.subjectProject nº 23222 (02/SAICT/2016)pt_PT
dc.subjectProject EXPOsEpt_PT
dc.titleExposure assessment in one central hospital: a multi-approach protocol to achieve an accurate risk characterizationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage108947pt_PT
oaire.citation.titleEnvironmental Researchpt_PT
oaire.citation.volume181pt_PT
person.familyNameViegas
person.familyNameAranha Caetano
person.familyNameCarolino
person.familyNameViegas
person.givenNameCarla
person.givenNameLiliana
person.givenNameElisabete
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id9716-9DAC-532A
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-1496-2609
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35270591500
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication6517c656-f913-4f54-8682-77c2856c9e4c
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscovery13115332-43f7-4048-a8a5-2f2b855a8c92

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