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Autoinhibition of TBCB regulates EB1-mediated microtubule dynamics

2013-01Journal article Open access
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dc.contributor.authorCarranza, Gerardo
dc.contributor.authorCastaño, Raquel
dc.contributor.authorFanarraga, Mónica L.
dc.contributor.authorVillegas, Juan Carlos
dc.contributor.authorGonçalves, João
dc.contributor.authorSoares, Helena
dc.contributor.authorAvila, Jesus
dc.contributor.authorMarenchino, Marco
dc.contributor.authorCampos-Olivas, Ramón
dc.contributor.authorMontoya, Guillermo
dc.contributor.authorZabala, Juan Carlos
dc.date.accessioned2013-06-25T13:27:10Z
dc.date.available2013-06-25T13:27:10Z
dc.date.issued2013-01
dc.description.abstractTubulin cofactors (TBCs) participate in the folding, dimerization, and dissociation pathways of the tubulin dimer. Among them, TBCB and TBCE are two CAP-Gly domain-containing proteins that together efficiently interact with and dissociate the tubulin dimer. In the study reported here we showed that TBCB localizes at spindle and midzone microtubules during mitosis. Furthermore, the motif DEI/M-COO− present in TBCB, which is similar to the EEY/F-COO− element characteristic of EB proteins, CLIP-170, and α-tubulin, is required for TBCE–TBCB heterodimer formation and thus for tubulin dimer dissociation. This motif is responsible for TBCB autoinhibition, and our analysis suggests that TBCB is a monomer in solution. Mutants of TBCB lacking this motif are derepressed and induce microtubule depolymerization through an interaction with EB1 associated with microtubule tips. TBCB is also able to bind to the chaperonin complex CCT containing α-tubulin, suggesting that it could escort tubulin to facilitate its folding and dimerization, recycling or degradation.por
dc.identifier.citationCarranza G, Castaño R, Fanarraga ML, Villegas JC, Gonçalves J, Soares H, et al. Autoinhibition of TBCB regulates EB1-mediated microtubule dynamics. Cell Mol Life Sci. 2013;70(2):357-71.por
dc.identifier.issn1420-9071
dc.identifier.urihttp://hdl.handle.net/10400.21/2578
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relation.publisherversionhttp://rd.springer.com/article/10.1007%2Fs00018-012-1114-2por
dc.subjectCell biologypor
dc.subjectBiomedicine generalpor
dc.subjectLife sciencespor
dc.subjectBiochemistrypor
dc.titleAutoinhibition of TBCB regulates EB1-mediated microtubule dynamicspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage371por
oaire.citation.startPage357por
oaire.citation.titleCellular and Molecular Life Sciencespor
oaire.citation.volume70por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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