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Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples

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In order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect Mycobacteria.

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Tuberculosis Mycobacteria Bacilli Bacteria Auramine Propidium iodide Cytometry

Citation

Pina-Vaz C, Costa-de-Oliveira S, Rodrigues AG, Salvador A. Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples. J Clin Microbiol. 2004;42(2):906-8.

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American Society for Microbiology

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