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- Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samplesPublication . Pina-Vaz, Cidália; Costa-de-Oliveira, Sofia; Rodrigues, Acácio G.; Salvador, AlexandreIn order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect Mycobacteria.