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Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin

2023-06Journal article Embargoed access
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dc.contributor.authorCanteiro, Beatriz
dc.contributor.authorMendes, Maria
dc.contributor.authorJacques, Filipa
dc.contributor.authorDelgadinho, Mariana
dc.contributor.authorOliveira, Ketlyn
dc.contributor.authorGinete, Catarina
dc.contributor.authorGomes, Mário
dc.contributor.authorRibeiro, Edna
dc.contributor.authorBrito, Miguel
dc.contributor.authorGomes, Anita Q.
dc.date.accessioned2023-08-23T11:41:06Z
dc.date.embargo2025-08-23
dc.date.issued2023-06
dc.descriptionIDI&CA-IPL/2022/miRCa/ESTeSLpt_PT
dc.description.abstractSickle Cell anemia (SCA) is a hereditary hemoglobinopathy with the formation of hemoglobin S, associated with severe health outcomes. Currently, induction of fetal hemoglobin (HbF) is one of the most promising therapeutic strategies. Here we aimed to assess the potential of the natural compound Quercetin, in transcriptional expression of globin and HbF regulatory/silencing genes. In this study, the K562 cell line was used as an SCA model. Cells were exposed to Quercetin at final concentrations of 0.2 and 20 µM, and Hydroxyurea (25 µg/mL) was used as a positive control. Cell viability and proliferation were assessed through trypan blue exclusion assay. Transcriptional expression was performed by RT-qPCR using specific primers. Significant differences were analyzed using a t-test. No cytotoxic effects were observed following exposure to Quercetin. The transcriptional analysis demonstrated that Quercetin affects mRNA levels of HbF regulatory/silencing genes with associated downregulation of BCL11A, MYB, KLF1, and HBB and upregulation of HBG and BGLT3, as well as alterations in the expression of miRNAs involved in HbF post-transcriptional regulation. Our results sustain Quercetin's potential as an HbF inducer with associated upregulation of HbF-activators and decreased expression of HbF-inhibitors. These data support the need for further studies in order to confirm the potential of this compound as a new therapeutic option for β -hemoglobinopathies in the future.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCanteiro B, Mendes M, Jacques F, Delgadinho M, Oliveira K, Ginete C, Gomes M, Ribeiro E, Brito M, Gomes AQ. Effects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin. In: ENBENG 2023 – 7th IEEE Portuguese Meeting on Bioengineering, Porto, June 22-23, 2023. p. 140-3.pt_PT
dc.identifier.doi10.1109/ENBENG58165.2023.10175363pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/16390
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationIDI&CA-IPL/2022/miRCa/ESTeSLpt_PT
dc.relation.publisherversionhttps://ieeexplore.ieee.org/document/10175363pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectSickle cell diseasept_PT
dc.subjectReactivation of fetal hemoglobinpt_PT
dc.subjectQuercetinpt_PT
dc.subjectFlavonoidpt_PT
dc.subjectHydroxyureapt_PT
dc.subjectHbF regulatory/silencing genespt_PT
dc.subjectIDI&CA-IPL/2022/miRCa/ESTeSLpt_PT
dc.titleEffects of quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobinpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage143pt_PT
oaire.citation.startPage140pt_PT
oaire.citation.titleENBENG 2023pt_PT
person.familyNameNeves Delgadinho
person.familyNameHonrado Ginete
person.familyNameRibeiro
person.familyNameBrito
person.familyNameGomes
person.givenNameMariana Isabel
person.givenNameAna Catarina
person.givenNameEdna
person.givenNameMiguel
person.givenNameAnita
person.identifierCAJ-5082-2022
person.identifier.ciencia-id231E-02E3-D9A9
person.identifier.ciencia-id8715-F62E-1E0F
person.identifier.ciencia-idC414-CDF2-D35A
person.identifier.ciencia-id231F-F341-7E93
person.identifier.ciencia-id4B10-E015-52B7
person.identifier.orcid0000-0003-0586-9154
person.identifier.orcid0000-0002-2334-782X
person.identifier.orcid0000-0003-1316-7750
person.identifier.orcid0000-0001-6394-658X
person.identifier.orcid0000-0002-3348-0448
person.identifier.ridA-7970-2016
person.identifier.ridC-3580-2014
person.identifier.scopus-author-id35224551000
person.identifier.scopus-author-id7202386033
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationca55aab6-9a58-4f79-ab79-20513414099f
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relation.isAuthorOfPublication.latestForDiscoveryca55aab6-9a58-4f79-ab79-20513414099f

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