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Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease

2022-04Journal article Open access
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dc.contributor.authorSilva, Inês
dc.contributor.authorSolas, João
dc.contributor.authorPinto, Rui
dc.contributor.authorMateus, Vanessa
dc.date.accessioned2022-04-27T16:12:52Z
dc.date.available2022-04-27T16:12:52Z
dc.date.issued2022-04
dc.descriptionIDI&CA_IPL/2019/COLITiS_ESTeSLpt_PT
dc.descriptionFCT_UIDB/05608/2020. FCT_UIDP/05608/2020.pt_PT
dc.description.abstractBackground: Inflammatory bowel disease (IBD) is a world healthcare problem. In order to evaluate the effect of new pharmacological approaches for IBD, we aim to develop and validate chronic trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. Methods: Experimental colitis was induced by the rectal administration of multiple doses of TNBS in female CD-1 mice. The protocol was performed with six experimental groups, depending on the TNBS administration frequency, and two control groups (sham and ethanol groups). Results: The survival rate was 73.3% in the first three weeks and, from week 4 until the end of the experimental protocol, the mice’s survival remained unaltered at 70.9%. Fecal hemoglobin presented a progressive increase until week 4 (5.8 ± 0.3 µmol Hg/g feces, p < 0.0001) compared with the ethanol group, with no statistical differences to week 6. The highest level of tumor necrosis factor-α was observed on week 3; however, after week 4, a slight decrease in tumor necrosis factor-α concentration was verified, and the level was maintained until week 6 (71.3 ± 3.3 pg/mL and 72.7 ± 3.6 pg/mL, respectively). Conclusions: These findings allowed the verification of a stable pattern of clinical and inflammation signs after week 4, suggesting that the chronic model of TNBS-induced colitis develops in 4 weeks.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva IJ, Solas J, Pinto R, Mateus V. Chronic experimental model of TNBS-induced colitis to study inflammatory bowel disease. Int J Mol Sci. 2022;23(9):4739.pt_PT
dc.identifier.doi10.3390/ijms23094739pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/14599
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationIDI&CA_IPL/2019/COLITiS_ESTeSLpt_PT
dc.relationFCT_UIDB/05608/2020pt_PT
dc.relationFCT_UIDP/05608/2020pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/9/4739pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectInflammatory bowel diseasept_PT
dc.subjectTNBS-induced colitispt_PT
dc.subjectChronic animal modelpt_PT
dc.subjectIDI&CA_IPL/2019/COLITiS_ESTeSLpt_PT
dc.subjectFCT_UIDB/05608/2020pt_PT
dc.subjectFCT_UIDP/05608/2020pt_PT
dc.titleChronic experimental model of TNBS-induced colitis to study inflammatory bowel diseasept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue9pt_PT
oaire.citation.startPage4739pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
person.familyNameda Silva
person.familyNamePinho Mateus
person.givenNameInês Filipa Janeiro da Silva
person.givenNameVanessa Alexandra
person.identifier.ciencia-idC010-323F-3266
person.identifier.ciencia-id5A12-571D-AD6A
person.identifier.orcid0000-0001-7049-2512
person.identifier.orcid0000-0002-3204-3772
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication995e1831-ff5b-49e7-a6e3-8bc692212204
relation.isAuthorOfPublication406041a5-682c-4f94-a4e2-ddbfc541313c
relation.isAuthorOfPublication.latestForDiscovery406041a5-682c-4f94-a4e2-ddbfc541313c

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