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Settleable dust and bioburden in Portuguese dwellings

2020-11Journal article Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorDias, Marta
dc.contributor.authorAlmeida, Beatriz
dc.contributor.authorVicente, Estela
dc.contributor.authorAranha Caetano, Liliana
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorAlves, Célia
dc.date.accessioned2020-12-05T21:56:08Z
dc.date.available2020-12-05T21:56:08Z
dc.date.issued2020-11
dc.descriptionUIDB/05608/2020. UIDP/05608/2020.pt_PT
dc.description.abstractMonitoring campaigns in several buildings have shown that occupants exposed to contaminated indoor air generally exhibit diverse health symptoms. This study intends to assess settleable dust loading rates and bioburden in Portuguese dwellings by passive sampling onto quartz fiber filters and electrostatic dust cloths (EDCs), respectively. Settled dust collected by EDCs was analyzed by culture-based methods (including azole-resistance screening) and qPCR, targeting four different toxigenic Aspergillus sections (Flavi, Fumigati, Circumdati, and Nidulantes). Dust loading rates and bioburden showed higher variability in the summer season. In both seasons, Penicillium sp. was the one with the highest prevalence (59.1% winter; 58.1% summer), followed by Aspergillus sp. in winter (13.0%). Fungal contamination increased in the winter period, while bacterial counts decreased. Aspergillus sections Circumdati and Nidulantes, detected in voriconazole supplemented media, and Aspergillus sections Fumigati and Nidulantes, detected by molecular tools, were found in the winter samples. This study reinforces the importance of applying: (a) Passive sampling methods in campaigns in dwellings; (b) two different culture media (MEA and DG18) to assess fungi; (c) in parallel, molecular tools targeting the most suitable indicators of fungal contamination; and (d) azole resistance screening to unveil azole resistance detection in fungal species.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Dias M, Almeida B, Vicente E, Caetano LA, Carolino E, et al. Settleable dust and bioburden in Portuguese dwellings. Microorganisms. 2020;8(11):1799.pt_PT
dc.identifier.doi10.3390/microorganisms8111799pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/12414
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationHealth and Technology Research Center
dc.relationHealth and Technology Research Center
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/8/11/1799pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectIndoor airpt_PT
dc.subjectAir qualitypt_PT
dc.subjectPassive sampling methodspt_PT
dc.subjectSettleable dustpt_PT
dc.subjectBioburdenpt_PT
dc.subjectAzole-resistance screeningpt_PT
dc.subjectAspergillus sp.pt_PT
dc.subjectUIDB/05608/2020pt_PT
dc.subjectUIDP/05608/2020pt_PT
dc.subjectPortugalpt_PT
dc.titleSettleable dust and bioburden in Portuguese dwellingspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth and Technology Research Center
oaire.awardTitleHealth and Technology Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05608%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F05608%2F2020/PT
oaire.citation.issue11pt_PT
oaire.citation.startPage1799pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameViegas
person.familyNameAranha Caetano
person.familyNameCarolino
person.givenNameCarla
person.givenNameLiliana
person.givenNameElisabete
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id9716-9DAC-532A
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-1496-2609
person.identifier.orcid0000-0003-4165-7052
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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