Publication
Immunohistochemistry in formalin-gel fixed tissues
| dc.contributor.author | Ferro, Amadeu Borges | |
| dc.contributor.author | Santos, Ana Bastos | |
| dc.contributor.author | Filipe, Joana Louro | |
| dc.date.accessioned | 2014-04-22T14:47:15Z | |
| dc.date.available | 2014-04-22T14:47:15Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | There are several hazards in histopathology laboratories and its staff must ensure that their professional activity is set to the highest standards while complying with the best safety procedures. Formalin is one of the chemical hazards to which such professionals are routinely exposed. To decrease this contact, it is suggested that 10% neutral buffered liquid formalin (FL) is replaced by 10% formalin-gel (FG), given the later reduces the likelihood of spills and splashes, and decreased fume levels are released during its handling, proving itself less harmful. However, it is mandatory to assess the effectiveness of FG as a fixative and ensure that the subsequent complementary techniques, such as immunohistochemistry (IHC), are not compromised. Two groups of 30 samples from human placenta have been fixed with FG and FL fixatives during different periods of time (12, 24, and 48 hours) and, thereafter, processed, embedded, and sectioned. IHC for six different antibodies was performed and the results were scored (0–100) using an algorithm that took into account immunostaining intensity, percentage of staining structures, non-specific immunostaining, contrast, and morphological preservation. Parametric and non-parametric statistical tests were used (alpha = 0•05). All results were similar for both fixatives, with global score means of 95•36±6•65 for FL and 96•06±5•80 for FG, and without any statistical difference (P>0•05). The duration of the fixation had no statistical relevance also (P>0•05). So it is proved here FG could be an effective alternative to FL. | por |
| dc.identifier.citation | Ferro AB, Santos AB, Filipe JL. Immunohistochemistry in formalin-gel fixed tissues. J Histotechnol. 2014;37(2):48-53. | por |
| dc.identifier.doi | 10.1179/2046023614Y.0000000040 | |
| dc.identifier.other | DOI: http://dx.doi.org/10.1179/2046023614Y.0000000040 | |
| dc.identifier.uri | http://hdl.handle.net/10400.21/3514 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Maney Online | por |
| dc.relation.publisherversion | http://www.maneyonline.com/doi/abs/10.1179/2046023614Y.0000000040?journalCode=his | por |
| dc.subject | Formalin gel | por |
| dc.subject | Fixation | por |
| dc.subject | Immunohistochemistry | por |
| dc.subject | Histopathology laboratory | por |
| dc.title | Immunohistochemistry in formalin-gel fixed tissues | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 53 | por |
| oaire.citation.issue | 2 | por |
| oaire.citation.startPage | 48 | por |
| oaire.citation.title | Journal of Histotechnology | por |
| oaire.citation.volume | 37 | por |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |
Files
Original bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- Immunohistochemistry in formalin_gel fixed tissues.pdf
- Size:
- 182.01 KB
- Format:
- Adobe Portable Document Format
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 1.71 KB
- Format:
- Item-specific license agreed upon to submission
- Description: