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Abstract(s)
Na presente dissertação são apresentados métodos de síntese para a obtenção de nanopontos de carbono (NPsC) luminescentes sustentáveis, utilizando como fonte de carbono águas residuais do processamento industrial do azeite (ARPIA) e demonstrada a sua aplicação direta como sensores de proteínas hémicas, e.g. hemoglobina (Hb).
Os NPsC foram sintetizados utilizando ARPIA provenientes de processos de extração contínuos (centrifugação, 2 fases (AR-2F) (ano 2016 (AR-2F-16) e ano 2017 (AR-2F-17)) e descontínuo (prensagem). A caracterização físico-química das ARPIA, assim como os diferentes métodos de síntese utilizados na obtenção dos NPsC são apresentados e discutidos ao longo deste trabalho.
Os NPsC sintetizados foram analisados e caracterizados estruturalmente por espetroscopia de infravermelho com transformada de Fourier (FTIR). Os NPsC sintetizados a partir de AR-2F foram também caracterizados por ressonância magnética nuclear de protão e carbono (RMN 1H e 13C), análise elementar (AE) e microscopia eletrónica de transmissão (TEM). Técnicas de absorção no estado fundamental (UV-Vis) e luminescência de estado estacionário foram aplicadas para o estudo das propriedades fotofísicas dos NPsC. A determinação do tempo de vida de luminescência dos NPsC obtidos a partir das AR-2F-17 foi igualmente efetuado.
A deteção de proteínas hémicas pelos NPsC provenientes das ARPIA foi comprovada. Os nanomateriais obtidos das AR-2F revelaram elevada sensibilidade e seletividade no reconhecimento de hemoglobina (Hb) em solução.
In the present work, synthetic methods to produce new fluorescent and sustainable carbon dots (CDs), using as carbon source olive mill waste waters (OMWWs), will be discussed, demonstrating their direct applicability as hemic protein sensors, e.g. haemoglobin (Hb). Different CDs were developed based on OMWWs of different origins, such as seasonal, i.e. OMWWs from the same process but differing in the harvest year (OMWW-2Ph-16 vs OMWW-2Ph-17) and processing methods (OMWW-2Ph-16/17 vs OMWWP). The main differences between these three types of OMWWs and the synthetic methods developed for each one of them are presented and discussed throughout. The CDs were analysed and structurally characterised by Fourier Transform Infrared spectroscopy (FTIR). The CDs that were synthesised by the OMWW-2Ph were additionally characterized by nuclear magnetic resonance of proton and carbon (1H and 13C NMR), elemental analysis (AE) and transmission electron microscopy (TEM). Absorption techniques at ground state and steady state luminescence were used for the study of photophysical properties. Luminescence lifetimes were studied using CDs from OMWW-2Ph-17. As a demonstration of the CDs applicability, it will be shown in this dissertation that these new CDs could be used as hemic proteins sensors. The study revealed a high sensibility and selectivity of the CDs towards haemoglobin.
In the present work, synthetic methods to produce new fluorescent and sustainable carbon dots (CDs), using as carbon source olive mill waste waters (OMWWs), will be discussed, demonstrating their direct applicability as hemic protein sensors, e.g. haemoglobin (Hb). Different CDs were developed based on OMWWs of different origins, such as seasonal, i.e. OMWWs from the same process but differing in the harvest year (OMWW-2Ph-16 vs OMWW-2Ph-17) and processing methods (OMWW-2Ph-16/17 vs OMWWP). The main differences between these three types of OMWWs and the synthetic methods developed for each one of them are presented and discussed throughout. The CDs were analysed and structurally characterised by Fourier Transform Infrared spectroscopy (FTIR). The CDs that were synthesised by the OMWW-2Ph were additionally characterized by nuclear magnetic resonance of proton and carbon (1H and 13C NMR), elemental analysis (AE) and transmission electron microscopy (TEM). Absorption techniques at ground state and steady state luminescence were used for the study of photophysical properties. Luminescence lifetimes were studied using CDs from OMWW-2Ph-17. As a demonstration of the CDs applicability, it will be shown in this dissertation that these new CDs could be used as hemic proteins sensors. The study revealed a high sensibility and selectivity of the CDs towards haemoglobin.
Description
Trabalho final de mestrado para obtenção do grau de mestre em Engenharia Química e Biológica
Keywords
Nanopontos de carbono luminescentes NPsC luminescentes Carbon dots CDs Águas residuais do processamento industrial do azeite () ARPIA Olive mil waste water OMWWs Fluorescência Fluorescence Hemoglobina Haemoglobin Hb conversão termoquímica Thermal conversion Recuperação de resíduos Waste recovery
Citation
SOUSA, Diogo Alexandre Cartaxo - Nanopontos de carbono a partir de resíduos industriais do processamento do azeite : produção e aplicações. Lisboa: Instituto Superior de Engenharia de Lisboa, 2018. Dissertação de mestrado.
Publisher
Instituto Superior de Engenharia de Lisboa