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Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry

2022-01Journal article Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorTwarużek, Magdalena
dc.contributor.authorDias, Marta
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorSoszczyńska, Ewelina
dc.contributor.authorCaetano, Liliana Aranha
dc.date.accessioned2022-01-26T13:16:37Z
dc.date.available2022-01-26T13:16:37Z
dc.date.issued2022-01
dc.descriptionFCT_EEA Grants Project “EXPO-Green”.pt_PT
dc.descriptionProject "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices” (IPL/2018/WasteFRPD_ESTeSL).pt_PT
dc.description.abstractSafe working conditions must be guaranteed during waste sorting, which is crucial to maximizing recycling and reuse, in order to minimize workers’ exposure to chemical and biological hazards. This study determines the contribution of Aspergillus section Fumigati to the overall cytotoxicity of filtering respiratory protection devices (FRPD) and mechanic protection gloves (MPG) collected in 2019 from different workstations in one waste sorting industry in Portugal. The cytotoxicity of 133 Aspergillus section Fumigati isolates was determined as IC50 in human A549 epithelial lung cells and swine kidney cells, using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Aspergillus section Fumigati cytotoxicity results were compared with previous total cytotoxicity data from FRPD and MPG samples. A significant correlation was detected between the total cytotoxicity of samples and cytotoxicity of Aspergillus section Fumigati isolates in A549 cells (rS = −0.339, p = 0.030). The cytotoxicity of Aspergillus section Fumigati isolates explained 10.7% of the total cytotoxicity of the sample. On the basis of the comparison of cytotoxicity levels, it was possible to determine the contribution of Aspergillus section Fumigati isolates for the total cytotoxicity of protection devices used in the waste sorting industry. The results support in vitro toxicology as a relevant approach in risk assessments regarding cytotoxicity in passive sampling, and thus, useful in determining the contribution of relevant microbial contaminants to overall cytotoxicity. This approach can provide valuable answers in dose/response studies, and support innovations in risk characterization and their translation into occupational policies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Twarużek M, Dias M, Carolino E, Soszczyńska E, Caetano LA. Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry. Toxins. 2022;14(2):70.pt_PT
dc.identifier.doi10.3390/toxins14020070pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/14208
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationFCT_EEA Grants Project “EXPO-Green”pt_PT
dc.relationProject "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices” (IPL/2018/WasteFRPD_ESTeSL).pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2072-6651/14/2/70pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectCitotoxicitypt_PT
dc.subjectProtection devicespt_PT
dc.subjectMTT assaypt_PT
dc.subjectRisk assessmentpt_PT
dc.subjectWaste sorting environmentpt_PT
dc.subjectFCT_EEA Grants Project “EXPO-Green”pt_PT
dc.subjectProject "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices”pt_PT
dc.subjectIPL/2018/WasteFRPD_ESTeSLpt_PT
dc.titleCytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industrypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue2pt_PT
oaire.citation.startPage70pt_PT
oaire.citation.titleToxinspt_PT
oaire.citation.volume14pt_PT
person.familyNameViegas
person.familyNameDias
person.familyNameCarolino
person.familyNameAranha Caetano
person.givenNameCarla
person.givenNameMarta
person.givenNameElisabete
person.givenNameLiliana
person.identifier2371423
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id4E1B-16BB-80C7
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-id9716-9DAC-532A
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0002-2704-6995
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1496-2609
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication564f2806-6e1e-418d-8d93-737922ad6601
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication6517c656-f913-4f54-8682-77c2856c9e4c
relation.isAuthorOfPublication.latestForDiscovery6517c656-f913-4f54-8682-77c2856c9e4c

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