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Opi1p translocation to the nucleus is regulated by hydrogen peroxide in Saccharomyces cerevisiae

2017-07Journal article Restricted access
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dc.contributor.authorCamelo, Carolina
dc.contributor.authorVilas-Boas, Filipe
dc.contributor.authorCepeda, Andreia Pereira
dc.contributor.authorReal, Carla
dc.contributor.authorBarros-Martins, Joana
dc.contributor.authorPinto, Francisco
dc.contributor.authorSoares, Helena
dc.contributor.authorMarinho, H. Susana
dc.contributor.authorCyrne, Luisa
dc.date.accessioned2017-08-21T16:55:24Z
dc.date.available2017-08-21T16:55:24Z
dc.date.issued2017-07
dc.description.abstractDuring exposure of yeast cells to low levels of hydrogen peroxide (H2 O2 ), the expression of several genes is regulated for cells to adapt to the surrounding oxidative environment. Such adaptation involves modification of plasma membrane lipid composition, reorganization of ergosterol-rich microdomains and altered gene expression of proteins involved in lipid and vesicle traffic, to decrease permeability to exogenous H2 O2. Opi1p is a transcriptional repressor that is inactive when present at the nuclear membrane/endoplasmic reticulum, but represseses transcription of inositol upstream activating sequence (UASINO )-containing genes, many of which are involved in the synthesis of phospholipids and fatty acids, when it is translocated to the nucleus. We investigated whether H2 O2 in concentrations inducing adaptation regulates Opi1p function. We found that, in the presence of H2 O2, GFP-Opi1p fusion protein translocates to the nucleus and, concomitantly, the expression of UASINO -containing genes is affected. We also investigated whether cysteine residues of Opi1p were implicated in the H2 O2 -mediated translocation of this protein to the nucleus and identified cysteine residue 159 as essential for this process. Our work shows that Opi1p is redox-regulated and establishes a new mechanism of gene regulation involving Opi1p, which is important for adaptation to H2 O2 in yeast cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCamelo C, Vilas-Boas F, Cepeda AP, Real C, Barros-Martins J, Soares H, et al. Opi1p translocation to the nucleus is regulated by hydrogen peroxide in Saccharomyces cerevisiae. Yeast. 2017;34(9):383-95.pt_PT
dc.identifier.doi10.1002/yea.3240pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/7344
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/yea.3240/abstractpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectRedox signallingpt_PT
dc.subjectHydrogen peroxidept_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectInositolpt_PT
dc.subjectOpi1ppt_PT
dc.subjectAdaptationpt_PT
dc.titleOpi1p translocation to the nucleus is regulated by hydrogen peroxide in Saccharomyces cerevisiaept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage395pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage383pt_PT
oaire.citation.titleYeastpt_PT
oaire.citation.volume34pt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT

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