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The expression of tubulin cofactor A (TBCA) is regulated by a noncoding antisense Tbca RNA during testis maturation

2012-08Journal article Open access
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dc.contributor.authorNolasco, Sofia
dc.contributor.authorBellido, Javier
dc.contributor.authorGonçalves, João
dc.contributor.authorZabala, Juan Carlos
dc.contributor.authorSoares, Helena
dc.contributor.authorTavares, Alexandra
dc.date.accessioned2013-01-29T15:23:29Z
dc.date.available2013-01-29T15:23:29Z
dc.date.issued2012-08
dc.description.abstractAbstract - Recently, long noncoding RNAs have emerged as pivotal molecules for the regulation of coding genes' expression. These molecules might result from antisense transcription of functional genes originating natural antisense transcripts (NATs) or from transcriptional active pseudogenes. TBCA interacts with β-tubulin and is involved in the folding and dimerization of new tubulin heterodimers, the building blocks of microtubules. Methodology/Principal findings: We found that the mouse genome contains two structurally distinct Tbca genes located in chromosomes 13 (Tbca13) and 16 (Tbca16). Interestingly, the two Tbca genes albeit ubiquitously expressed, present differential expression during mouse testis maturation. In fact, as testis maturation progresses Tbca13 mRNA levels increase progressively, while Tbca16 mRNA levels decrease. This suggests a regulatory mechanism between the two genes and prompted us to investigate the presence of the two proteins. However, using tandem mass spectrometry we were unable to identify the TBCA16 protein in testis extracts even in those corresponding to the maturation step with the highest levels of Tbca16 transcripts. These puzzling results led us to re-analyze the expression of Tbca16. We then detected that Tbca16 transcription produces sense and natural antisense transcripts. Strikingly, the specific depletion by RNAi of these transcripts leads to an increase of Tbca13 transcript levels in a mouse spermatocyte cell line. Conclusions/Significance: Our results demonstrate that Tbca13 mRNA levels are post-transcriptionally regulated by the sense and natural antisense Tbca16 mRNA levels. We propose that this regulatory mechanism operates during spermatogenesis, a process that involves microtubule rearrangements, the assembly of specific microtubule structures and requires critical TBCA levels.por
dc.identifier.citationNolasco S, Bellido J, Gonçalves J, Tavares A, Zabala JC, Soares H. The expression of tubulin cofactor A (TBCA) is regulated by a noncoding antisense Tbca RNA during testis maturation. PLoS ONE. 2012;7(8):e42536.por
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.21/2083
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherPLoSpor
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0042536por
dc.subjectAmino acid sequencepor
dc.subjectAnimalspor
dc.subjectBase sequencepor
dc.subjectCell linepor
dc.subjectChromosomes, mammalian/geneticspor
dc.subjectGene expression regulation, developmentalpor
dc.subjectGene knockdown techniquespor
dc.subjectGenome/geneticspor
dc.subjectMalepor
dc.subjectMicepor
dc.subjectMicrotubule-associated proteins/chemistrypor
dc.subjectMicrotubule-associated proteins/geneticspor
dc.subjectMicrotubule-associated proteins/metabolismpor
dc.subjectModels, molecularpor
dc.subjectMolecular chaperones/chemistrypor
dc.subjectMolecular chaperones/geneticspor
dc.subjectMolecular chaperones/metabolismpor
dc.subjectMolecular sequence datapor
dc.subjectRNA, Antisense/geneticspor
dc.subjectRNA, Antisense/metabolismpor
dc.subjectRNA, Messenger/geneticspor
dc.subjectRNA, Messenger/metabolismpor
dc.subjectRNA, Untranslated/geneticspor
dc.subjectRNA, Untranslated/metabolismpor
dc.subjectSpermatocytes/metabolismpor
dc.subjectSpermatogenesis/geneticspor
dc.subjectTestis/growth & developmentpor
dc.subjectTestis/metabolismpor
dc.subjectTranscription, Geneticpor
dc.titleThe expression of tubulin cofactor A (TBCA) is regulated by a noncoding antisense Tbca RNA during testis maturationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPagee42536por
oaire.citation.titlePLoS ONEpor
oaire.citation.volume7por
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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