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Detection of TR34/L98H pan-azole-resistant Aspergillus fumigatus in poultry farm environments

2026-08Artigo científico Acesso aberto
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datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorGomes, Bianca
dc.contributor.authorDias, Marta
dc.contributor.authorCervantes, Renata
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorRamos, Miguel J. N.
dc.contributor.authorNunes, Mónica
dc.contributor.authorDias, Ricardo
dc.contributor.authorViegas, Carla
dc.date.accessioned2026-09-01T11:43:41Z
dc.date.available2026-09-01T11:43:41Z
dc.date.issued2026-08
dc.descriptionThe author(s) declared that financial support was received for this work and/or its publication. This work was supported by FCT – Fundação para a Ciência e Tecnologia, I.P., through project UI/BD/153746/2022 and the CE3C unit UID/00329/2025. It was also supported by project 2023.01366.BD.
dc.description.abstractIntroduction: Poultry farms have been recognized as environments prone to fungal contamination. However, the occurrence of azole-resistant Aspergillus fumigatus and its cytotoxic potential remain insufficiently characterized. This study aimed to characterize the occurrence, cytotoxic potential, and azole-resistance profile of Aspergillus section Fumigati in poultry farms.Methods: A total of 420 samples, including air (n = 47), electrostatic dust cloths (n = 87), bedding (n = 87), feed (n = 95), swabs (n = 87), workers’ masks (n = 2), and broiler breast (n = 15), were obtained. Fungal characterization was performed through culture-based methods (27 °C and 37 °C), followed by azole resistance screening according to EUCAST guidelines. Resistant isolates were subjected to whole-genome sequencing and a targeted analysis of cyp51A mutations. The cytotoxicity potential of fungal isolates and environmental samples was evaluated using selected cell lines representing respiratory organs (human alveolar [A549]) and detoxification organs (swine kidney [SK]/hepatocellular carcinoma [HepG2]). Results: Seven putative Aspergillus fumigatus isolates exhibited pan-azole resistance (MICs: ≥ 2 mg/L ITR/VOR; ≥ 1mg/L for POS). Four isolates carried the TR34/L98H mutation and were recovered from bedding (n = 3) and air (n = 1), suggesting the presence of resistant A. fumigatus in poultry farm matrices. Although 12% of isolates induced toxicity on both cell lines (17/145), no significant association was observed between isolate cytotoxicity and environmental sample toxicity, suggesting that additional biological and chemical components may contribute to the overall toxicological profile of farm environments. Discussion: The study highlights the occurrence of azole-resistant A. fumigatus in poultry farms and supports integrated surveillance approaches addressing antifungal resistance and environmental exposure risks in poultry production.eng
dc.description.sponsorshipThe author(s) declared that financial support was received for this work and/or its publication. This work was supported by FCT – Fundação para a Ciência e Tecnologia, I.P., through project UI/BD/153746/2022 (https://doi.org/10.54499/UI/BD/153746/2022) and the CE3C unit UID/00329/2025 (https://doi.org/10.54499/UID/00329/2025). It was also supported by project 2023.01366.BD (https://doi.org/10.54499/2023.01366.BD).
dc.identifier.citationGomes B, Dias M, Cervantes R, Gonçalves L, Carolino E, Viegas C, et al. Detection of TR34/L98H pan-azole-resistant Aspergillus fumigatus in poultry farm environments. Front Fungal Biol. 2026;7:1858050.
dc.identifier.doi10.3389/ffunb.2026.1858050
dc.identifier.issn2673-6128
dc.identifier.urihttp://hdl.handle.net/10400.21/23033
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media SA
dc.relation.hasversionhttps://www.frontiersin.org/journals/fungal-biology/articles/10.3389/ffunb.2026.1858050/full
dc.relation.ispartofFrontiers in Fungal Biology
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAspergillus fumigatus
dc.subjectAzole-resistance profile
dc.subjectBedding material
dc.subjectcyp51A gene mutations
dc.subjectCytotoxic potential
dc.subjectOne health
dc.subjectFCT_UI/BD/153746/2022
dc.subjectCE3C unit UID/00329/2025
dc.subject2023.01366.BD
dc.titleDetection of TR34/L98H pan-azole-resistant Aspergillus fumigatus in poultry farm environmentseng
dc.typejournal article
dcterms.referenceshttps://www.frontiersin.org/articles/10.3389/ffunb.2026.1858050/full#supplementary-material
dspace.entity.typePublication
oaire.citation.startPage1858050
oaire.citation.titleFrontiers in Fungal Biology
oaire.citation.volume7
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGomes
person.familyNameRosado Silva Dias
person.familyNameWellenkamp de Carvalho Freire de Cervantes
person.familyNameCarolino
person.familyNameViegas
person.givenNameBianca
person.givenNameMarta Sofia
person.givenNameRenata
person.givenNameElisabete
person.givenNameCarla
person.identifier.ciencia-idAD1E-B4CE-389D
person.identifier.ciencia-id0E13-5F70-9D8A
person.identifier.ciencia-id6018-3CC9-B640
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.orcid0000-0002-6264-6072
person.identifier.orcid0000-0001-6797-7711
person.identifier.orcid0000-0001-5990-4168
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0002-1545-6479
person.identifier.ridB-7217-2013
person.identifier.scopus-author-id55443609700
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