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Validação de método de análise quantitativa de carvacrol por HPLC-DAD em creme veterinário de Origanum vulgare L. 2% (m/m)
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Advisor(s)
Abstract(s)
Introdução – A utilização de cremes contendo óleos essenciais de plantas aromáticas é uma área em crescente desenvolvimento em medicina veterinária. Os óleos essenciais apresentam propriedades biológicas, incluindo atividades antimicrobianas, antioxidantes e anti-inflamatórias que podem coadjuvar o tratamento de problemas de pele em gatos e cães. O controlo de qualidade de um creme com óleo essencial de Origanum vulgare L. inclui, entre outros ensaios, a quantificação de carvacrol (um monoterpeno fenólico maioritário). Objetivos – Pretende-se extrair, separar e quantificar o carvacrol presente num creme farmacêutico, validando este método de controlo de qualidade. Métodos – Foram realizadas três extrações sólido-líquido consecutivas de 100mg de creme, tendo-se obtido uma distribuição da quantidade de carvacrol extraída de 74,7%, 19,4% e 5,9%, respetivamente, correspondendo a 95% de recuperação do total de óleo essencial contido no creme. Os extratos foram analisados por HPLC-DAD numa coluna RP-C18 com uma fase móvel de metanol:água:acetonitrilo. Resultados – O método foi validado, tendo resultado em precisão intraensaio e interensaio inferiores a 2%RSD. Obteve-se limite de deteção de 0,65µg/mL e limite de quantificação de 1,95µg/mL. O carvacrol constituía cerca de 36% da massa do óleo essencial do quimiotipo principal. Conclusões – O método desenvolvido pode ser utilizado para controlo de qualidade de cremes contendo carvacrol, podendo ser aplicado a outros monoterpenos.
ABSTRACT Introduction – The use of creams containing essential oils from aromatic plants is a growing area in veterinary medicine. Essential oils contain biological properties, including antimicrobial, antioxidant, and anti-inflammatory activities, which can support the treatment of skin problems in cats and dogs. Quality control of creams containing Origanum vulgare L. essential oil includes, among other analyses, quantification of carvacrol, a major phenolic monoterpene. Objectives – This study aims to extract, separate, and quantify the carvacrol present in a cream formulation, thereby validating this quality control method. Methods – Three consecutive solid-liquid extractions were performed on 100mg of cream, yielding a distribution of extracted carvacrol quantities of 74.7%, 19.4%, and 5.9%, respectively. This corresponded to a total recovery of 95% of the essential oil content in the cream. The extracts were analyzed by HPLC-DAD on an RP-C18 column with a mobile phase of methanol:water:acetonitrile. Results – The method was validated, resulting in intra-assay and inter-assay precision values below 2% RSD. The LOD was found to be 0.65µg/mL, and the LOQ was 1.95µg/mL. Carvacrol constituted approximately 36% of the mass of the main chemotype's essential oil. Conclusions – The validated method is suitable for quality control of creams containing carvacrol and may be adapted for analysis of other monoterpenes.
ABSTRACT Introduction – The use of creams containing essential oils from aromatic plants is a growing area in veterinary medicine. Essential oils contain biological properties, including antimicrobial, antioxidant, and anti-inflammatory activities, which can support the treatment of skin problems in cats and dogs. Quality control of creams containing Origanum vulgare L. essential oil includes, among other analyses, quantification of carvacrol, a major phenolic monoterpene. Objectives – This study aims to extract, separate, and quantify the carvacrol present in a cream formulation, thereby validating this quality control method. Methods – Three consecutive solid-liquid extractions were performed on 100mg of cream, yielding a distribution of extracted carvacrol quantities of 74.7%, 19.4%, and 5.9%, respectively. This corresponded to a total recovery of 95% of the essential oil content in the cream. The extracts were analyzed by HPLC-DAD on an RP-C18 column with a mobile phase of methanol:water:acetonitrile. Results – The method was validated, resulting in intra-assay and inter-assay precision values below 2% RSD. The LOD was found to be 0.65µg/mL, and the LOQ was 1.95µg/mL. Carvacrol constituted approximately 36% of the mass of the main chemotype's essential oil. Conclusions – The validated method is suitable for quality control of creams containing carvacrol and may be adapted for analysis of other monoterpenes.
Description
Ao Politécnico de Lisboa, instituição financiadora do projeto PLANT-VET, através da bolsa IPL/IDI&CA2024/PLANT-VET_ESTeSL. Aos finalistas da Licenciatura em Farmácia Gonçalo Silva, João Cabaço, Mafalda Silva e Rita Cotrim, pela relevante colaboração no projeto PLANT-VET, que permitiu a obtenção do creme utilizado.
Keywords
Carvacrol Origanum vulgare L. Creme HPLC-DAD Controlo de qualidade Cream Quality control IPL/IDI&CA2024/PLANT-VET_ESTeSL
Pedagogical Context
Citation
Pádua M, Machado A, Zegre M, Martinho P, Costa-Veiga A. Validação de método de análise quantitativa de carvacrol por HPLC-DAD em creme veterinário de Origanum vulgare L. 2% (m/m). Saúde & Tecnologia. 2025;(31):e953. Online first.
Publisher
Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa