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The comet assay for human biomonitoring: effect of cryopreservation on DNA damage in different blood cell preparations

2019-07Journal article Restricted access
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dc.contributor.authorLadeira, Carina
dc.contributor.authorKoppen, Gudrun
dc.contributor.authorScavone, Francesca
dc.contributor.authorGiovannelli, Lisa
dc.date.accessioned2019-03-22T15:13:31Z
dc.date.available2019-03-22T15:13:31Z
dc.date.issued2019-07
dc.descriptionCOST Action hCOMET 15132 for supporting this research with a Short-Term Scientific Mission (STSM 38872)pt_PT
dc.description.abstractThis study was designed within the frame of the COST Action hCOMET 15132 (Working Group 6), with the aim of comparing different peripheral blood cell preparations for their feasibility in human biomonitoring studies, using the comet assay for the evaluation of DNA damage. Basal levels of strand breaks/ALS and formamidopyrimidine DNA glycosylase (Fpg) - sites, and H2O2 (500 μM)-induced strand breaks, were measured in whole blood, peripheral blood mononuclear cells - lymphocytes and monocytes - and buffy coat; in fresh and 1, 4 and 12 weeks-frozen samples. The comparison among the fresh preparations showed that the basal levels of DNA damage were all very low and similar in the three samples. Frozen whole blood samples stored in cryostraws without cryoprotection showed similar basal levels of DNA damage as fresh samples, indicating that this preparation, often chosen for biobanks, resists efficiently freezing/thawing artifacts. However, long-term storage of frozen buffy coat samples in cryostraws and with no cryopreservative did not appear feasible. Storage up to 3 months of frozen cryoprotected peripheral blood mononuclear cells induced small increases in basal strand breaks and no other statistically significant modification. Altogether, this study suggests that whole blood could be the most suitable sample to be used to perform comet assay in human epidemiological biomonitoring for genotoxicity assessment in frozen samples, such as those stored in biobanks.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLadeira C, Koppen G, Scavone F, Giovannelli L. The comet assay for human biomonitoring: effect of cryopreservation on DNA damage in different blood cell preparations. Mutat Res Genet Toxicol Environ Mutagen. 2019;843:11-7.pt_PT
dc.identifier.doi10.1016/j.mrgentox.2019.02.002pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/9755
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCOST Action hCOMET 15132pt_PT
dc.relationShort-Term Scientific Mission (STSM 38872)pt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1383571818302274?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectWhole bloodpt_PT
dc.subjectBuffy coatpt_PT
dc.subjectPeripheral blood mononuclear cellspt_PT
dc.subjectFreezingpt_PT
dc.subjectStoragept_PT
dc.subjectDNA damagept_PT
dc.subjectComet assaypt_PT
dc.titleThe comet assay for human biomonitoring: effect of cryopreservation on DNA damage in different blood cell preparationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage17pt_PT
oaire.citation.startPage11pt_PT
oaire.citation.titleMutation Research - Genetic Toxicology and Environmental Mutagenesispt_PT
oaire.citation.volume843pt_PT
person.familyNameLadeira
person.givenNameCarina
person.identifier144237
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.orcid0000-0001-5588-0074
person.identifier.ridJ-2572-2012
person.identifier.scopus-author-id36463788000
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1aef4b60-4197-436b-84ab-80d31cbaed33
relation.isAuthorOfPublication.latestForDiscovery1aef4b60-4197-436b-84ab-80d31cbaed33

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