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Aspergillus flavus contamination in two Portuguese wastewater treatment plants

2014-07Journal article Restricted access
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dc.contributor.authorViegas, Carla
dc.contributor.authorDias, R.
dc.contributor.authorGomes, Anita Q.
dc.contributor.authorMeneses, Márcia
dc.contributor.authorSabino, Raquel
dc.contributor.authorViegas, Susana
dc.date.accessioned2014-08-05T15:38:14Z
dc.date.available2014-08-05T15:38:14Z
dc.date.issued2014-07
dc.description.abstractFilamentous fungi from genus Aspergillus were previously detected in wastewater treatment plants (WWTP) as being Aspergillus flavus (A. flavus), an important toxigenic fungus producing aflatoxins. This study aimed to determine occupational exposure adverse effects due to fungal contamination produced by A. flavus complex in two Portuguese WWTP using conventional and molecular methodologies. Air samples from two WWTP were collected at 1 m height through impaction method. Surface samples were collected by swabbing surfaces of the same indoor sites. After counting A. flavus and identification, detection of aflatoxin production was ensured through inoculation of seven inoculates in coconut-milk agar. Plates were examined under long-wave ultraviolet (UV; 365 nm) illumination to search for the presence of fluorescence in the growing colonies. To apply molecular methods, air samples were also collected using the impinger method. Samples were collected and collection liquid was subsequently used for DNA extraction. Molecular identification of A. flavus was achieved by real-time polymerase chain reaction (RT-PCR) using the Rotor-Gene 6000 qPCR detection system (Corbett). Among the Aspergillus genus, the species that were more abundant in air samples from both WWTP were Aspergillus versicolor (38%), Aspergillus candidus (29.1%), and Aspergillus sydowii (12.7%). However, the most commonly species found on surfaces were A. flavus (47.3%), Aspergillus fumigatus (34.4%), and Aspergillus sydowii (10.8%). Aspergillus flavus isolates that were inoculated in coconut agar medium were not identified as toxigenic strains and were not detected by RT-PCR in any of the analyzed samples from both plants. Data in this study indicate the need for monitoring fungal contamination in this setting. Although toxigenic strains were not detected from A. flavus complex, one cannot disregard the eventual presence and potential toxicity of aflatoxins.por
dc.identifier.citationViegas C, Dias R, Gomes AQ, Meneses M, Sabino R, Viegas S. Aspergillus flavus contamination in two Portuguese wastewater treatment plants. J Toxicol Environ Health A. 2014;77(14-16):796-805.por
dc.identifier.doi10.1080/15287394.2014.909300
dc.identifier.urihttp://hdl.handle.net/10400.21/3727
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherTaylor & Francispor
dc.relation.publisherversionhttp://www.tandfonline.com/doi/full/10.1080/15287394.2014.909300#.U-DulbGG7qEpor
dc.subjectEnvironmental healthpor
dc.subjectSurface contaminationpor
dc.subjectAspergillus flavuspor
dc.subjectWastewater treatment plantspor
dc.subjectFungal contaminationpor
dc.subjectPortugalpor
dc.titleAspergillus flavus contamination in two Portuguese wastewater treatment plantspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage805por
oaire.citation.startPage796por
oaire.citation.titleJournal of Toxicology and Environmental Health. Part Apor
oaire.citation.volume77por
person.familyNameViegas
person.familyNameSabino
person.familyNameViegas
person.givenNameCarla
person.givenNameRaquel Filipa Pinheiro
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0001-6585-7775
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id35796067600
person.identifier.scopus-author-id35270591500
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublicationcf64684a-fb92-4421-91da-14fb15462fad
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscovery13115332-43f7-4048-a8a5-2f2b855a8c92

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